血管内皮生长因子和醛糖还原酶对糖尿病大鼠股骨头结构和微血管形态的影响  

Effect of VEGF and AR activity upon femoral head structure and micrangium morphology of diabetic rats

作　　者：陈海英[1] 郑建盛[1] 陈建明[1] 陈少强[2] 刘文革[3] 张鹏飞[4] 

机构地区：[1]福建莆田学院医学院基础医学部,福建莆田351100 [2]福建医科大学人体解剖学与组织胚胎学系,福州350003 [3]福建医科大学附属协和医院骨科,福州350003 [4]福建医科大学病理学系,福州350003

出　　处：《解剖学报》2009年第1期130-136,共7页Acta Anatomica Sinica

基　　金：福建省教育厅基金资助项目(JA08189)

摘　　要：目的观察糖尿病大鼠股骨头微血管分布及形态上的变化。探讨糖尿病大鼠股骨头组织血管内皮生长因子（VEGF）mRNA在不同时间表达的差异以及醛糖还原酶（AR）活性变化对股骨头组织结构演变、微血管病变的影响。方法建立速发型链脲佐菌素（STZ）糖尿病大鼠模型，随机分为正常5周组（CON1）、10周组（CON2）及15周组（CON3），糖尿病5周组（DM1）、10周组（DM2）及15周组（DM3），每组10只。光镜下观察各组股骨头结构以及凝血因子Ⅷ免疫组织化学表达情况；墨汁灌注行微血管密度测定；观察VEGFmRNA原位杂交表达强度并分析；生化检测各组股骨头组织匀浆的All活性。结果糖尿病大鼠股骨头随病程发展骨小梁变少变薄，骨髓腔增大；凝血因子Ⅷ因子表达上升，微血管密度增大；VEGFmRNA在骨髓腔血管内皮细胞表达上升，AR活性增大（P〈0．05）。结论糖尿病大鼠股骨头出现骨质疏松变化倾向，与VEGFmRNA促进微血管增生、血管通道性增大及AR活性增强密切相关。Objective To observe the distribution and morphologie variation of micrangium in diabetic rat＇ s femoral head and to probe into the difference of the expression of VEGF mRNA at the different time and the effect of the variation of the aldose reductase（AR） activity on the femoral head structure as well as mierangium lesion. Methods To set up the diabetic rat＇s model with STZ, we divided the rats into three normal groups： CON1, CON2 and CON3 and three diabetic groups： DMI, DM2 and DM3, ten rats in each group. The structure of femoral heads of different group rats was observed under light microscope. The expression of coagulation factor ~ was detected with immunohistochemistry. The density of micrangium was measured by infusion with ink. We also observed the expressive intenstiy of VEGF mRNA in situ hybridization and analysised the activity of AR by making biochemical detection of the tissue homogenate of femoral head of the rat in each group. Results The diabetic rats＇ femoral heads developed with the course of illness, the bone trabecula became thin and few, the marrow cavities enlarged. The expression of coagulation factor ~ rose, the microvessel density enlarged. The expression of VEGF mRNA in vascular of the marrow cavities endothelial cells rose, and the activity of AR was enhancement （ P 〈 0.05 ）. Conclusion In the early period of diabetic rat＇s femoral head the inclining changes of osteoporosis occured. This is positive correlation with microvessel hyperplasia, increased permeability and enhancement of the activity of AR promoted by the VEGF mRNA.

关 键 词：糖尿病 股骨头 微血管 血管内皮生长因子 醛糖还原酶 墨汁灌注 原位杂交 大鼠 

分 类 号：R587.1[医药卫生—内分泌]