还原叶酸载体基因多态性与宫颈癌关系的病例对照研究  

A case-control study on the association of RFC-1 polymorphism and cervical cancer

作　　者：弟娟娟[1] 王金桃[2] 丁玲[2] 王志敏[3] 高尔生[1] 

机构地区：[1]上海市计划生育科学研究所,上海200032 [2]山西医科大学流行病学教研室 [3]安徽省疾病预防控制中心

出　　处：《中华流行病学杂志》2009年第2期189-191,共3页Chinese Journal of Epidemiology

基　　金：山西省自然科学基金资助项H（2008011075-1）

摘　　要：目的探讨叶酸代谢相关酶基因还原叶酸载体基因（RFC-1）多态性与宫颈癌易感性的关系以及与人乳头瘤病毒（HPV）16在宫颈癌发生中的交互作用。方法采用以医院为基础的频数匹配病例对照研究，选择经病理学确诊的宫颈鳞状细胞癌新发病例107例作为病例组，子宫肌瘤患者107例作为对照组，应用特异性PCR和限制性片段长度多态性（RFLP）分别检测HPV16及RFC.1基因A80G多态性。结果（1）HPV16感染率病例组（56.07％）高于对照组（31.78％），调整OR值为3.25（95％CI：1.74-6.08）。（2）病例组与对照组各基因型频率符合Hardy-Weinberg平衡，病例组RFC-1基因80AA、AG和GG各基因型频率在病例组和对照组间差异有统计学意义（X^2=6.66，P=0.03）。A80G等位基因在两组的分布存在差异（X^2=7.41，P=0.01）。（3）RFC-1基因A80G多态性与宫颈癌易感性的分析结果显示，与携带AA基因型者相比，携带GG基因型者患宫颈癌的危险性增高（OR=2.42，95％CI：1.01-5.81）。（4）RFC-1基因多态性与HPVl6感染经logistic回归模型分析，在宫颈癌发生中交互作用无统计学意义。结论RFC，1基因A80G位点的纯合型（GG）可增加宫颈癌发生的危险。Objective To evaluate the possible association between RFC-1 polymorphism and cervix carcinoma, as well as the interaction between polymorphism and human papilloma virusl6 （HPV16）. Methods Based on a hospital-based case-control study, 107 cases which were diagnosed as cervical cancer pathematologically and 107 controls with hysteromyoma, were selected by frequency, matched with age and habitation. HPV16 and RFC-1 A80G polymorphism were detected by special PCR and RFLP. Results （1）HPVI6 infection rate in cases （56.07%） was higher than that in controls （31.78%） with the adjusted OR of 3.25 （95%CI： 1.74-6.08）. （2） All the genetypes were tested by Hardy-Weinberg balance. （3） Compared with RFC-1 AA, RFC-1 GG had higher risk for cervical cancer with OR of 2.42 （95%CI： 1.01-5.81 ）. （4） No statistical significance was noticed regarding the interaction between RFC-1 polymorphism and HPV16 in logistic regression method. Conclusion The introduction of RFC-1 80GG gene type could increase the risk of cervical cancer.

关 键 词：宫颈肿瘤 病例对照研究 RFC-1基因多态性 

分 类 号：R737.33[医药卫生—肿瘤]