傅里叶变换红外光谱与核磁共振波谱对结直肠癌诊断价值的初步研究  

Fourier Transform Infrared Spectrometry and Magnetic Resonance Spectrometry for the Diagnosis of Colorectal Cancer

作　　者：姚宏伟[1] 高秀香[2] 赵梅仙[2] 张元福[2] 付卫[1] 徐智[1] 石雪迎[3] 徐怡庄[2] 

机构地区：[1]北京大学第三医院普通外科,北京100191 [2]北京大学化学与分子工程学院稀土材料化学及应用国家重点实验室,北京100871 [3]北京大学医学部病理学系,北京100191

出　　处：《中国微创外科杂志》2009年第1期59-61,共3页Chinese Journal of Minimally Invasive Surgery

基　　金：国家自然科学基金资助项目(批准号:50673005)

摘　　要：目的探讨傅里叶变换红外光谱（fourier transform infrared spectrometry,FTIRS）、核磁共振波谱（magneticresonance spectroscopy,MRS）应用于临床结直肠癌离体标本分析的可行性,探索其用于结直肠癌临床诊断的新途径。方法2007年3月-2008年4月,对47例新鲜、离体的结直肠正常黏膜和癌组织标本,进行病理学检查,并应用FTIRS、MRS进行分析并研究结直肠正常黏膜与癌组织之间的差异。结果以病理学结果作为参照,FTIRS和MRS判定正常黏膜以及癌组织的诊断准确率分别为94.7%（89/94）和97.8%（45/46）,敏感率分别为93.6%（44/47）和100%（23/23）,特异率分别为95.7%（45/47）和95.7%（22/23）,假阳性率4.2%（2/47）和4.3%（1/23）,假阴性率6.4%（3/47）和0%（0/23）,阳性预测值95.7%（44/46）和95.8%（23/24）,阴性预测值93.8%（45/48）和100%（22/22）。结论FTIRS、MRS能够快速、准确地鉴别结直肠组织的良、恶性,有可能发展成为微创、早期、快捷诊断结直肠癌的方法。Objective To analyze ex vivo samples of colorectal cancers by Fourier transform infrared spectrometry and magnetic resonance spectrometry, and to explore the feasibility to diagnose the tumor by using the methods in clinic. Methods From March 2007 to April 2008, fresh samples colorectal mucosa and carcinoma were obtained from 47 patients. The regimens were examined pathologically and then analyzed by Fourier transform infrared spectrometry and magnetic resonance spectrometry. The accuracy of the spectrometrical results was determined by comparing with the pathological results. Results The accuracy of the Fourier transform infrared spectrometry and magnetic resonance spectrometry was 94. 7% （89/94） and 97.8% （45/46） , respectively, while the sensitivity was 93.6% （44/47） and 100% （23/23） , specificity was 95.7% （45/47） and 95.7% （22/23） , false positive rate was 4.3% （2/47） and 4.3% （1/23） , false negative rate was 6.4% （3/47） and 0% （0/23） , positive prognostic value was 95.7% （44/46） and 95.8% （23/24） , and the negative prognostic value was 93.8% （45/48） and 100% （22/22）. Conclusions Benign and malignant colorectal tissues can be identified quickly and accurately by Fourier transform infrared spectrometry and magnetic resonance spectrometry. The methods, which are minimally invasive, could be a potential diagnosing tool for colorectal cancer at an early stage.

关 键 词：红外光谱 核磁共振波谱 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]