家兔胸腰段前侧脑脊液漏模型的建立  

A Method of Establishing Experimental Model of Thoracolumbar Anterior Dural Injury Cerebrospinal Fluid Leakage in Rabbit

作　　者：文睿[1] 王清[1] 何仁建[1] 

机构地区：[1]泸州医学院附属医院脊柱外科,四川泸州646000

出　　处：《中国现代医生》2009年第3期10-11,14,F0003,共4页China Modern Doctor

摘　　要：目的建立能长期存活的家兔胸腰段前侧脑脊液漏实验模型。方法健康成年新西兰家兔32只,雌雄不限,体重1.75～2.5kg,普通环境饲养,3～6月龄,随机分为四组。均作胸腹联合切口。切除12肋,推开腰大肌,显露T12～L2椎体左侧壁,分离L1腰动脉,切断并结扎,充分显露椎体后,行L1椎体次全切,显露硬膜囊。每组动物分别制造胸腰前路术中不同类型的脊膜损伤类型:A组:单纯线性脊膜切开0.5cm,B组:单纯线性脊膜切开1.0cm,C组:脊膜缺损0.25cm2,D组:脊膜缺损0.5cm2。术后30d行椎管造影CT扫描,美蓝灌注试验及病理学检查。结果A组:脊膜均愈合;B组:脊膜愈合4例,假性脊膜囊肿形成2例,漏口缩小但未闭合2例;C组:脊膜愈合2例,漏口缩小但未闭合3例,假性脊膜囊肿形成2例;D组:漏口缩小但未闭合5例,假性脊膜囊肿形成1例。各组均可见脊膜淋巴细胞、浆细胞等炎性细胞浸润,脊膜变厚。可见非坏死性炎性浸润。脊膜原切口处有瘢痕组织形成。结论经胸腹联合切口,切除12肋,显露T12￣L2椎体左侧壁、分离,切断并结扎L1腰动脉后,行L1椎体次全切,显露硬膜囊,制造不同类型的脊膜损伤,是建立能长期存活的胸腰段前侧脑脊液漏模型简便、安全可靠的方法。可为后续研究提供实验依据。Objective To establish a long-term surviving model of thoracolumbar anterior dural injury cevebrospinal fluid leakage in rabbit. Methods Establish an animal model which include 32 rabbits. Those animals were randomly divided into four groups with 8 rabbits each. After Ketamine Anesthesia,adopt abdomino-thoracic incision,burst through musculi psoas,removal 12th rib,expose side wall of T12-L2 vertebra,truncate and ligate L1 arteriae lumbales. Subtotal resect L1 vertebrae,expose dural mater. Make different thoracolumbar dural mater injury in each groups. Group A：simplicity linear dural mater incision of 0.5cm, Group B：simplicity linear dural matter incision of lcm, Group C：dural mater defect of 0.5cm^2, GroupD：dural mater defect of 1 cm^2. gelfoam and hemostatic gauze were used to wadded the dural mater leakage,BFS is used too. All animals have cerebrospinal fluid from drainage tube. 30 days after surgery. CT spinal cannal myelography,methylene blue test, and dural matter pathologic examination were tested. Results Group A：all rabbits dural matter wound healed. Group B ：4 cases dural mater healed, 1 case found meningeal cyst, 1 case dural mater incision reduce. Group C：2 case dural matter healed, 3 cases dural mater incision reduce,2 case found meningeal cyst. Group D：5 cases dural mater incision reduce, 1 case found meningeal cyst. In each group,we can see homeoeyte, phlogocyte etc inflammatory cell infiltrate, dural mater thicken, non-necrotic inflammatory infiltration, dural mater incision cicatrization. Conclusion It is safe,convenient and credible to establish a model of thoracolumbar anterior dural injury cerebrospinal fluid leakage in rabbit by adopting abdominothoracic incision, removing 12th rib,exposing side wall of T12-L2 vertebra,truncating and ligating L1 arteriae lumbales,subtotal resecting L1 vertebrae,exposing dural mater and making different thoracolumbar dural matter injury in rabbit. It can provide an experimental foundation to subsequent research.

关 键 词：胸腰段 脊膜损伤 脑脊液漏 动物模型 

分 类 号：R-332[医药卫生]