COX-2、β-cat、MMP-7表达与遗传性非息肉病性大肠癌特殊侵袭转移行为的关系  

Cyclooxgenase-2,β-catenin and matrix metalloproteinase-7 expression and their correlation with invasion/metastasis behaviours of hereditary nonpolyposis colorectal cancer

作　　者：顾国利[1] 王石林[1] 魏学明[1] 任力[2] 熊梅[2] 胡益云[2] 李德昌[2] 邹福先[3] 成健[4] 

机构地区：[1]中国人民解放军空军总医院普通外科,北京市100142 [2]中国人民解放军空军总医院病理科,北京市100142 [3]江西理工大学附属校医院外科,江西省赣州市341000 [4]中国人民解放军空军总医院手术室,北京市100142

出　　处：《世界华人消化杂志》2009年第2期151-157,共7页World Chinese Journal of Digestology

摘　　要：目的:本研究旨在探讨COX-2、β-cat和MMP-7表达与HNPCC特殊侵袭转移生物学行为间的关系.方法:应用免疫组化SP法检测HNPCC(n=28)、散发性大肠癌(n=30)和正常大肠黏膜(n=10)中COX-2,β-cat和MMP-7的表达情况.所有标本预先经过hMSH2和hMLH1免疫组化染色筛查,并结合其临床病理特点进行回顾性分析.结果:在HNPCC组和散发性大肠癌组中COX-2、异位β-cat和MMP-7的表达差异显著(χ2=14.8352,P=0.0001;χ2=5.6425,P=0.0175;χ2=10.6454,P=0.0011).两组异位β-cat和MMP-7的阳性率与大肠癌的侵袭深度和淋巴结转移密切相关(P=0.0127,0.0001;P=0.0227,0.0261),而与性别、肿瘤的大小和部位无关.COX-2在HNPCC组中与肿瘤侵袭深度相关(P=0.0166),与淋巴结转移无关;散发性大肠癌组中与肿瘤侵袭深度和淋巴结转移均无关.两组中COX-2、异位β-cat和MMP-7三者阳性表达呈正相关(COX-2与异位β-cat:r=0.417,P=0.011,r=0.504,P=0.006;异位β-cat与MMP-7:r=0.396,P=0.027,r=0.429,P=0.021;COX-2与MMP-7:r=0.315,P=0.028,r=0.429,P=0.021).结论:COX-2、异位β-cat和MMP-7在HNPCC、散发性大肠癌和正常黏膜中的阳性表达率差异显著,这可能是HNPCC相对于散发性大肠癌侵袭弱、转移少的原因之一.AIM： To detect cyclooxgenase-2 （COX-2）, β-catenin （β-cat） and matrix metalloproteinase-7 （MMP-7） expression in hereditary nonpolyposis colorectal cancer （HNPCC） and sporadic colorectal carcinoma （CRC）, and to analyze their relationship with the biological behaviour of HNPCC. METHODS： The SP Immunohistochemical staining was used to detect COX-2, β-cat and MMP-7 protein expression in sample tissues of 28 HNPCC, 30 sporadic CRC and 10 normal colorectal cancer. All of the specimens were selected beforehand by hMSH2 and hMLH1 Immunohistochemical staining. And their corresponding clinical data were analyzed retrospectively. RESULTS： The positive expression rates of COX-2, β-cat in cytoplasm and MMP-7 expression differed significantly between HNPCC and sporadic CRC （X^2 = 14.8352, P = 0.0001; X^2 = 5.6425, P =0.0175; x^2 = 10.6454, P = 0.0011）. Positive rates of malposed β-cat and MMP-7 were closely correlated with the neoplastic invasive depth in HNPCC group and sporadic CRC group （P = 0.0127, P = 0.0001; P = 0.0227, P = 0.0261） and lymph node metastasis （P = 0.0000, P = 0.0001; P = 0.0227, P = 0.0261）, but not with the sex, the size or position of the tumour. COX-2 expression was bound up with the neoplastic invasive depth （P = 0.0166） in HNPCC group, but not with the lymph node metastasis. However, in sporadic CRC group, COX-2 expression was related with neither neoplastic invasive depth nor lymph node metastasis. There was a stable positive relationship among COX-2, malposed β-cat and MMP-7 expression both in HNPCC and sporadic CRC （COX-2 and malposed β-cat： r = 0.417, P = 0.011, r = 0.504, P = 0.006; malposed β-cat and MMP-7： r = 0.396, P = 0.027, r = 0.429, P = 0.021; COX-2 and MMP-7： r = 0.315, P = 0.028, r = 0.429, P = 0.021）. CONCLUSION： The present study demonstrates that the COX-2,β-cat in cytoplasm and MMP-7 expression has marked difference among HNPCC, sporadic CRC and normal colorectal tissues. This may be an important reason why HNPCC tumors h

关 键 词：遗传性非息肉病性大肠病 人类错配修复基因 环氧合酶-2 Β-链接素 基质金属蛋白酶-7 病理 免疫组化 转移 治疗 

分 类 号：R735.34[医药卫生—肿瘤] R735.2[医药卫生—临床医学]