外源性一氧化碳对小肠缺血再灌注大鼠多形核中性粒细胞聚集及血浆TNF-α、IL-10的影响  

Effects of exogenous carbon monoxide on polymorphonuclear neutrophil, TNF-α and IL-10 expression in rats with intestinal ischemiareperfusion injury

作　　者：韩宁[1] 李勇 陈卫民[1] 

机构地区：[1]中国医科大学附属盛京医院麻醉科,辽宁省沈阳市110004 [2]辽河油田妇婴医院麻醉科,辽宁省盘锦市124000

出　　处：《世界华人消化杂志》2009年第1期74-77,共4页World Chinese Journal of Digestology

摘　　要：目的:通过检测小肠缺血再灌注(IIR)大鼠不同组织内多形核中性粒细胞(PMN)聚集及血浆肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)浓度的变化,探讨外源性CO对IIR所致多器官损伤防治作用的机制.方法:♂Wistar大鼠64只,随机分为8组,给予不同实验方法处理.实验结束时取不同组织光镜下计数PMN聚集情况,取动脉血放免法检测血浆TNF-α和IL-10浓度.结果:肠、肺和肝组织中PMN数目在各组存在差异(66±6vs60±4,55±3,49±4,42±4,37±3,30±2,26±2;52±5vs43±5,42±4,34±2,32±2,25±2,23±2,18±2;35±3vs30±3,26±1,23±2,20±1,19±1,16±1,均P<0.05);肾组织中各组PMN数目无明显差异.各组血浆TNF-α、IL-10存在明显差异(3.15±0.05vs2.37±0.14,2.07±0.07,1.89±0.07,1.69±0.09,1.53±0.06,1.31±0.06,1.15±0.04;138.9±11.37,118.75±7.69,100.55±4.86,83.12±5.13,61.41±6.88,40.56±2.85vs23.55±4.94,23.55±4.9415vs36±2.37,均P<0.05).结论:外源性CO对IIR所致器官损伤的防治作用可能是通过抑制IIR过程中PMN在组织中的聚集,抑制TNF-α产生和促进IL-10释放实现的.AIM： To investigate the effects of carbon monoxide （CO） on multiple organ injury induced by intestinal ischemia-reperfusion （IIR） in rats. METHODS： Sixty-four male Wistar rats were randomly allocated to eight groups with eight in each group. Different groups were handlinged. The numbers of PMN in different tissues were observed at light microscopy, and the plasma TNF-α, IL-10 concentrations were determined using radio-immunifaction method. RESULTS： Marked differences in PMN of intestine, lung and liver were detected among groups, but not in kidney （66 ± 6 vs 60 ± 4, 55 ± 3, 49 ± 4, 42 ± 4, 37 ± 3, 30 ± 2, 26 6± 2; 52 ± 5 vs 43 ± 5, 42 ± 4, 34 ± 2, 32 ± 2, 25 ± 2, 23 ± 2, 18 ± 2; 35 ± 3 vs 30 ± 3, 26 ± 1, 23 ± 2, 20 ± 1, 19 ±1, 16 ± 1, all P〈 0.05）. Significant differences in TNF-α, IL-10 were detected among all group （3.15 ± 0.05 vs 2.37 ± 0.14, 2.07 ± 0.07, 1.89 ± 0.07, 1.69 ± 0.09, 1.53 ± 0.06, 1.31 ± 0.06, 1.15 ± 0.04; 138.9 ± 11.37, 118.75 ± 7.69, 100.55 ± 4.86, 83.12 ± 5.13, 61.41 ±6.88, 40.56 ± 2.85 vs 23.55 ± 4.94, 23.55 ± 4.9415 vs 36 ± 2.37, all P 〈 0.05）. CONCLUSION： Exogenous CO provides protection against multiple organ injury induced by IIR, partly through inhibiting PMN aggregation in tissues, inhibiting the elevation of TNF-α and promoting the release of IL-10.

关 键 词：一氧化碳 小肠 缺血再灌注 多形核中性 粒细胞 肿瘤坏死因子-α 白细胞介素-10 

分 类 号：R574[医药卫生—消化系统]