胆囊占位病变中P53和K-ras的表达及其临床意义  

Expression of P53 and K-ras in benign neoplasms and carcinoma of gallbladder

作　　者：王延召[1] 于则利[2] 张立军[2] 

机构地区：[1]北京大学首钢医院普外二科,北京市100044 [2]首都医科大学附属同仁医院普外科,北京市100730

出　　处：《世界华人消化杂志》2009年第1期94-96,共3页World Chinese Journal of Digestology

基　　金：北京市优秀人才资助项目;No2005~~

摘　　要：目的:研究P53和K-ras蛋白在胆囊良性占位病变和胆囊癌组织中的表达及差异,探讨其临床意义.方法:应用免疫组织化学方法检测20例胆固醇息肉、22例胆囊腺瘤、25例胆囊癌(含有5例重度非典型增生原位癌)组织中P53和K-ras蛋白的表达,进行统计学分析.结果:胆囊良性病变的黏膜组织中存在P53和K-ras的异常表达,其中,胆囊腺瘤患者的阳性表达显著高于胆囊胆固醇息肉患者(72.7%vs35.0%,P=0.014;90%vs75.0%,P=0.167);胆固醇性息肉患者的P53和K-ras表达较低.配对比较的P53和K-ras表达结果无差异,两者可能在胆囊癌的发生过程中有协同性作用.结论:胆固醇性息肉、胆囊腺瘤、胆囊癌患者的病变组织中P53和K-ras蛋白的表达有显著性差异,表达阳性率呈现上升趋势.AIM： To detect the expression of P53 and K-ras in gallbladder benign lesions and malignant diseases, and to examine their clinical significance. METHODS： Twenty specimens with cholesterol polyps, 22 with adenoma of gallbladder, 25 with primary gallbladder cancer （PGC） （including 5 with atypical hyperplasia and carcinoma in situ） were examined in this study for P53 and K-ras＇ expressions using immunohistochemistry. A statistical analysis was performed. RESULTS： Aberrant expression of P53 and K-ras was present in benign lesions. The positive rate was significantly higher in patients with adenoma of gallbladder than with cholesterol polyps （72.7% vs 35.0%, P = 0.014; 90% vs 75.0%, P = 0.167）. Expression of P53 and K-ras was the lowest in patients with cholesterol polyps. Paired comparison between P53 and K-ras showed no significant difference, which indicated a possible synergistic interaction in development of gall- bladder cancer. CONCLUSION： Expression of P53 and K-ras shows significant difference and its positive rates are on the increase among cholesterol polyps, adenoma of gallbladder and primary gallbladder cancer （PGC）.

关 键 词：胆囊癌 胆囊腺瘤 胆固醇息肉 P53 K—ras 

分 类 号：R575.6[医药卫生—消化系统]