杂交信号放大方法快速检测结核分枝杆菌的实验研究  

Study on Rapid Detection of M.tuberculosis by Hybridization Signal Amplification Method

作　　者：王海河[1] 赵春燕[1] 李凡[1] 

机构地区：[1]吉林大学白求恩医学院病原生物学教研室,吉林长春130021

出　　处：《中国实验诊断学》2009年第2期176-178,共3页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助课题(30672007)

摘　　要：目的确定杂交信号放大方法(HSAM)检测结核分枝杆菌的灵敏度和特异性,进而探讨该方法用于检测临床标本的可行性。方法HSAM法检测合成的IS6110靶基因、结核分枝杆菌标准菌株、大肠埃希菌和表皮葡萄球菌,确定该方法的灵敏度和特异性,并与PCR法进行比较。结果HSAM最少能检测到10合成的靶基因和结核分枝杆菌,与PCR方法灵敏度相近,标准菌株检测结果阳性,大肠埃希菌和表皮葡萄球菌均为阴性,该方法具有较高的特异性。结论HSAM具有高灵敏度、高特异性及操作简便快速等特点,可作为结核分枝杆菌临床标本检测方法。Objective To determine the sensitivity and specificity of hybridization signal amplification method（HSAM） for detection M. tuberculosis and to determine the feasibibility in detection M. Tuberculosis clinical specimens. Methods The sensitivity and specificity of HSAM were compared with those of PCR by detection synthetic IS6110, M. tuberculosis swain standards H37Rv, E. eoli and S. epidermidis. Results The lowest number of targets and M. tuberculosis detected by HSAM were10 molecules, the M. tuberculosis strain standards, E. coli and S. epidermidis were tested, the results showed that M. tuberculosis strain standards was positive, while E. coli and S. epidennidis were negative. Conclusion HSAM could be used for the deefion of M. Tuberculosis in clinical specimens because of its high sensitivity and high specificity.

关 键 词：杂交信号 结核分枝杆菌 HSAM 

分 类 号：R450[医药卫生—治疗学]