双血管抑制基因通过不同治疗方法对大鼠肝癌的抑瘤效果分析  

Effect of combining the genes of endostatin and angiostatin in hepatoma of wistar rat

作　　者：谢耀萍[1] 杨志杰[1] 李勇[1] 马铎[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院核医学科,黑龙江哈尔滨150001

出　　处：《中国实验诊断学》2009年第2期179-181,共3页Chinese Journal of Laboratory Diagnosis

基　　金：黑龙江省教育厅资助项目(11521194);黑龙江省卫生厅科研课题(2007-234)

摘　　要：目的分析局部注射和肝动脉给予血管抑素基因(Angiostatin)和内皮抑素基因(Endostatin)重组质粒对大鼠肝癌治疗效果的差异。方法将肝癌模型大鼠随机分为:局部治疗组、局部对照组、肝动脉治疗组、肝动脉对照组。治疗组给予200μL质粒和脂质体复合物和对照组给予生理盐水200μL。治疗后观测肿瘤大小、微血管密度、血管内皮生长因子、肿瘤凋亡指标的变化。结果6d开始基因治疗组各治疗指标与对照组相比均具有统计学差异,(P<0.05-0.001)。局部治疗组与肝动脉治疗组相比也具有统计学意义,(P<0.05-0.01)。结论本研究的结果显示,重组质粒两种方式对于肝癌均有抑制效果,但肝动脉的抑瘤效果不如局部注射,主要与重组质粒进入肿瘤方式不同有关。Objective To analyse the therapeutic action of Angiostatin and Endostain gene in the hepatoma and region injection of rots. Methods Hepatic cancer models were randomly divided into 4 groups that include 2 control groups and 2 therapy groups. In the region therapy,200 μL two gene plasmids（20 μg） and 200μL physiologic saline were locally injected in hepatic cancer. In the hepatic artery groups, the same plasmids and physiologic saline were gave the hepafie model. The voluminal growth rote, cancer tissular microvessel density（MVD）, vascular endothelial growth factor（VEGF）, apoptotie index（AI） were measured in each group. Results Each gene therapy group index owned the statistic difference to control group（ P 〈 0.05- 0.001）;At the same time,comparing with the region gene therapy group,and the hepatic therapy groups also had the statistic significance （ P 〈 0.05- 0.01）. Conclusion The two therapy methods have effect in the hepatic cancer. The effect of region therapy is better than the hepatic artery. The results were created by the blood would dilute the plasmas dose and the plasmas can＇ t Stay the region long time.

关 键 词：肝癌模型 基因治疗 血管抑素基因 内皮抑素基因 

分 类 号：R735.7[医药卫生—肿瘤]