化学-酶法制备^(15)N-L-酪氨酸的研究  

Study on preparation of stable isotope Iabeled ^(15)N-L-tyrosine by chemical-enzymatic way

作　　者：侯静华[1] 徐大刚[1] 徐建飞[1] 杜晓宁[1] 庄英萍[2] 宋明鸣[1] 

机构地区：[1]上海化工研究院,上海200062 [2]华东理工大学,上海200237

出　　处：《化学工程师》2009年第2期10-13,共4页Chemical Engineer

基　　金：上海市科委基金项目(O6dz22207)(;O6dz11304)

摘　　要：通过化学-酶法途径制备了稳定性同位素标记的15N-L-酪氨酸。以大肠杆菌所含的天冬氨酸转氨酶催化自制的对羟基苯丙酮酸和15N-L-天冬氨酸,转化成15N-L-酪氨酸。主要对酶转化的条件进行了研究,酶反应液经分离纯化后,得到的15N-L-酪氨酸产品的丰度达98.62%,比原料下降了0.44%,15N的利用率达40.78%。^15N-L-tyrosine was prepared by means of biotransformation of p-hydroxy phenylpyruvic acid and ^15N-L-aspartic acid, which made by ourselves, in the presence of aspartate aminotransferase from E.coli.The convetted liquid was separated and purified, and ^15N-L-tyrosine was obtained. The high abundance test gave fairly good results： the enrichment of ^15N-L-tyrosine was 98.62%, 0.44% decline as compared to the raw material-^15N-L-aspartic acid, and the utilization rate of ^15N was 40.78%.

关 键 词：15N-L-酪氨酸 天冬氨酸转氨酶 稳定性同位素 制备 

分 类 号：TQ464.7[化学工程—制药化工]