非小细胞肺癌外周血癌胚抗原mRNA表达与肿瘤微血管密度的相关性  

Relationship between expression of carcinoembryonic antigen mRNA in peripheral blood and microvessel density of tumor in non-small cell lung cancer

作　　者：王祖义[1] 汪国文[1] 刘学刚[1] 唐震[1] 史向前[1] 李小军[1] 刘以尧[1] 段贵新[1] 周晓[1] 贡会源[1] 

机构地区：[1]蚌埠医学院第一附属医院胸心外科,安徽蚌埠233004

出　　处：《蚌埠医学院学报》2009年第2期100-102,共3页Journal of Bengbu Medical College

基　　金：安徽省教育厅自然科学研究计划项目资助(2002kj220)

摘　　要：目的:探讨非小细胞肺癌(NSCLC)外周血癌胚抗原(CEA)mRNA的表达与肿瘤微血管密度(MVD)之间的相关性。方法:采用逆转录巢式聚合酶链反应(nestedRT-PCR)和微流控芯片(micro-fluidchip)技术检测57例NSCLC患者术前外周血CEAmRNA;选择单克隆抗体CD31和CD34,采用免疫组化对肿瘤组织进行MVD计数。结果:57例NSCLC患者外周血CEAmRNA阳性表达率为61.4%;CD31和CD34显示的MVD结果分别为(29.7±12.1)条和(38.2±12.7)条,范围分别是10～61条和19～76条。CEAmRNA阳性表达率和MVD计数呈正相关关系(P<0.05)。结论:在NSCLC患者,其肿瘤MVD较高的则更容易发生外周血微转移。Objective：To investigate the relationship between microvessel density （MVD） of the tumor and earcinoembryonic antigen （CEA） mRNA expression of peripheral blood （PB） in patients with non-small cell lung cancer （NSCLC）. Methods：Nested reverse transcription polymerase chain reaction （nested RT-PCR） and micro-fluid chip were used to detect CEA mRNA from PB;ElivisionTM plus immunohistochemieal technique was used to investigate the expression of CD31 and CD34. Results：In the 57 cases of NSCLC,the positive rate of CEA mRNA was 61.4% and the MVDs shown by CD31 and CD34 were 29.7 ± 12.1 and 38.2 ± 12.7,respectively, within a range of 10 -61 and 19 -76 . The expression of CEA mRNA was positively related to MVD （P 〈 0.05 ）. Conclusions：For patients with NSCLC,high MVD may increase the chances of micrometastasis.

关 键 词：癌 非小细胞肺 癌胚抗原 微血管密度 微转移 微流控芯片 

分 类 号：R734.2[医药卫生—肿瘤]