吐温80合并温热对B16黑色素瘤细胞hsp 70、c-fos、泛蛋白及S-100蛋白表达的影响  

INFLUENCE OF HYPERTHERMIA (41 C ) IN COMBINATION WITH TWEEN-80 ON EXPRESSION OF HSP 70, C-FOS. UBIQUITIN AND S-100 PROTEIN IN B16 MELANOMA CELL LINE

作　　者：杨耀琴[1] 陶惠红[1] 朴文姬[1] 杨虎川[1] 

机构地区：[1]上海铁道大学基础医学院肿瘤细胞研究室,上海200070

出　　处：《解剖学杂志》1998年第2期127-131,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(39170825);上海市科技发展基金(BB03801)资助

摘　　要：目的:研究吐温80与温热合并的协同抗肿瘤机理及与凋亡的关系.方法:用免疫组化方法.观察吐温80合并41℃温热作用后即时、4小时和72小时,B16肿瘤细胞热休克蛋白70(hsp 70)、c-fos、泛蛋白以及S-100蛋白表达的改变,并进行定量分析.结果:hsp70和C-fos蛋白呈中等阳性反应,分别位于B16细胞的胞质和胞核;泛蛋白为弱阳性、S-100蛋白呈强阳性,分布于胞质;41℃ 60分钟作用可增强hsp70表达.以4小时为显著.核亦呈阳性反应;对其它蛋白表达无明显影响;吐温80单独可轻度抑制hsp70表达;温热与吐温80合并作用能显著抑制hsp70表达,c-fos和泛蛋白表达则明显增加.S-100蛋白变化各组无显著意义.结论:吐温80增强温热抗肿瘤的效应可能与其抑制hsp70合成.影响细胞热耐受性的产生和诱导细胞调亡有关.B16 Melanoma cell line was treated by Tween-80 and hyperthermia at 41 C for 60 min. The expression and distribution of hsp70. c-fos. ubiquitin and S-100 protein were studied by immuno-cytochemistry at 0 hour. 4 hour and 72 hour respectively after the treatment. The results showed that B16 cells expressed all hsp 70. c-fos. ubiquitin and S-100 protein at 37 C in different levels. They were mainly located in the cytoplasm with exception of c-fos. Hyperthermia (41 C for 60 min) could increase the expression of hsp70 in B16 cells markedly. There was a peak at 4 hour and the cell nuclei also presented positive response. Other proteins did not change by hyperthermia (P>0. 05). Tween-80 alone decreased hsp70 but increased ubiquitin expression slightly. Tween-80 and hyperthermia at 41 C inhibited hsp70 expression significantly (P<0.01). but increased c-fos and ubiquitin expression obviously (P<0. 05. P<0. 01). S-100 protein was stable and was not affected by Tween-80 and hyperthermia. These results suggested that synergetic anti-tumor effect of Tween-80 with heating could be involved in inhibiting the thermotolerance and inducing apoptosis of B16 melanoma cells.

关 键 词：黑色素瘤 热休克蛋白70 泛蛋白 温热疗法 

分 类 号：R739.505[医药卫生—肿瘤] R730.59[医药卫生—临床医学]