异氟醚对大鼠脑缺血/再灌注诱导c-Jun磷酸化的影响  

Effect of isoflurane on focal cerebral ischemia/reperfusion-induced phosphorylation of c-Jun in rats

作　　者：温相如[1] 关秋华[2] 苗蓓[3] 张光毅[2] 

机构地区：[1]徐州医学院化学教研室,江苏徐州221002 [2]徐州医学院江苏省脑病生物信息重点实验室 [3]徐州医学院江苏省麻醉学重点实验室

出　　处：《徐州医学院学报》2009年第2期71-73,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金(90608015);江苏省教育厅资助项目(06KJB180113)

摘　　要：目的观察大鼠脑缺血/再灌注后c-Jun蛋白磷酸化情况以及异氟醚对其的影响。方法四动脉结扎法建立大鼠脑缺血模型。随机分为假手术组、直接脑缺血/再灌注组、吸入2 h 1.5 MAC异氟醚脑缺血/再灌注组和吸入纯氧2 h脑缺血/再灌注组,全脑缺血15 min后分别再灌注6 h。再灌注6 h的大鼠进行磷酸化c-Jun(p-c-Jun)的免疫印迹和免疫组化检测。结果缺血前吸入1.5 MAC异氟醚组大鼠的c-Jun磷酸化水平明显低于直接脑缺血/再灌注组和吸入纯氧2 h脑缺血/再灌注组(P<0.05)。结论异氟醚抑制c-Jun蛋白的磷酸化可能是其对大鼠缺血/再灌注性脑损伤产生保护作用的机制之一。Objective To observe the focal cerebral ischemia/reperfusion - induced phosphorylation of c - Jun in rats and investigate the effect of isoflurane. Methods Animal model of global cerebral ischemia was established by ligation of bilateral common carotid and vertebral arteries （ four vessels）. Healthy male Sprague - Dawley rats were randomly divided into four groups： sham operation group （S） , ischemia/reperfusion group （I/R） , 2 h 1.5 MAC isoflurane preconditioning ＋ ischemia/reperfusion group （ I/R ＋ Iso） and 2 h pure O2 inhalation ＋ ischemia/reperfusion group （ I/R ＋ O2 ）. 15 rain after global cerebral ischemia, the rats were allowed 6 hours of reperfusion. Determination of phosphoryla- tion of c - Jun was performed by Western blotting and immunohistochemistry. Results The phosphorylation levels of c - Jun in I/R ＋ Iso group was markedly lower than that in I/R and I/R ＋ O： groups （ P 〈 0.05 ）. Conclusion Isoflurane is able to inhibit the phosphorylation of c - Jun, which might be one of its protective mechanisms in the ischemia/reperfusion -induced brain injuries in rats.

关 键 词：脑 缺血/再灌注损伤 异氟醚 p-c-Jun 

分 类 号：R743.31[医药卫生—神经病学与精神病学]