铜锌超氧化物歧化酶基因对HepG2肝癌细胞增殖的抑制作用  

THE EFFECT OF CuZnSOD GENE ON INHIBITION OF THE PROLIFERATIONOF HepG2 HEPATOCELLULAR CANCER CELLS

作　　者：白景香[1] 祝学光[1] 郑新程[1] 伍贻经 

机构地区：[1]北京医科大学人民医院外科

出　　处：《北京医科大学学报》1998年第2期121-123,共3页Journal of Peking University(Health Sciences)

摘　　要：目的：探讨超氧化物歧化酶（ＳＯＤ）对肝癌细胞增殖的抑制作用。方法：利用逆转录病毒载体介导的基因转移技术，将人铜锌ＳＯＤ基因导入人肝癌细胞系ＨｅｐＧ２细胞内，获得高表达。检测其对ＨｅｐＧ２细胞生物学特性的影响。结果：与对照相比ＳＯＤ基因转染后的肝癌细胞生长受到抑制，细胞体积变大，分裂象减少，Ｓ期细胞比率减少，软琼脂集落形成率低，裸鼠成瘤瘤体小。结论：铜锌ＳＯＤ基因具有抑制ＨｅｐＧ２肝癌细胞增殖的作用。Objective: To explore the effect of superoxide dismutase (SOD)on inhibition of HepG2 hepatocellular cancer cell proliferation. Methods:Using gene transfer technique, hepatocellular cancer cells(HepG2) were transfected with retroviral vector containing human CuZnSOD cDNA and the elevated SOD gene expression was compared with the parental and neo control cells. The biological characteristics of the cells were measured. Results:Compared with the control cells, cancer cells transfected with SOD gene showed an inhibited growth rate,an increased cell mass, a reduced number of cells in S phase and a decreased clone forming ability in soft agar as well as a smaller tumor size formed in nude mice. Conclusion: The CuZnSOD gene overexpression could inhibit the proliferation of HepG2 hepatocellular cancer cells.

关 键 词：肝肿瘤 超氧化物歧化酶 药理学 肿瘤抑制 

分 类 号：R735.705[医药卫生—肿瘤] R979.1[医药卫生—临床医学]