重组人骨形成蛋白-2/珊瑚复合人工骨的研制及其诱导成骨的实验研究  

An experimental study on the preparation of recombinant human bone morphogenetic protein-2/coral composited artificial bone and its osteoinductivity

作　　者：张森林[1,2,3] 毛天球[1,2,3] 王会信 赵明[1,2,3] 

机构地区：[1]第四军医大学口腔医院 [2]南京市南京军区总医院口腔科 [3]军事医学科学院基础医学研究所

出　　处：《实用口腔医学杂志》1998年第2期97-99,共3页Journal of Practical Stomatology

摘　　要：目的：制备珊瑚（ｃｏｒａｌ）与重组人骨形成蛋白－２（ｒｈＢＭＰ－２）复合人工骨并测试其骨诱导活性。方法：将ｒｈＢＭＰ－２与珊瑚复合后植入鼠肌袋内。６６只小鼠随机分为３组：第１组为复合骨（１∶２０ｗ／ｗ）；第２组为复合骨（１∶４０ｗ／ｗ）；第３组为单独珊瑚。术后１、３、６周处死动物，光镜下观察，并用图象分析法作成骨定量测定，结果：术后１周，可见复合材料表面及孔洞内有软骨形成。３周出现编织骨。６周形成含骨髓的板层骨。珊瑚被部分吸收。诱导成骨的量有时间依赖性和ｒｈＢＭＰ－２剂量依赖性。结论：ｒｈＢＭＰ－２／ｃｏｒａｌ复合人工骨具有良好的诱导成骨能力；珊瑚可能是目前能使用的ｒｈＢＭＰ－２最合适的缓释载体。Objective:To prepare recombinant human morphogenetic protein 2 (rhBMP 2)/coral composite attificial bone and to determine its osteoinductivity.Methods:The composite artificial bone was prepared by combining coral with rhBMP-2,and was implanted into the muscle pouches of mice.Sixty six mices were divited into three groups:Group 1, the composite bone (coral:rhBMP 2(w/w)=20∶1)transplanted;Group 2, the composite bone (coral∶rhBMP 2(w/w)=40∶1);and Group 3 the coral alone.All the animals were killed at intervals of 1,3,5 weeks after operation.The speciments were evaluated by light microscope and image analysis.Results:Cartilage growth in the pores or on the surface of the implants was observed one week after operation, woven bone three weeks and lamellar bone with bone marrow six weeks,partial coral absorption was observed,the amount of induced endochondral bone was time and rhBMP 2 dose dependent.Conclusion:The results indicate that the composite possesses a superior osteoinductivity and coral may act as one of the most suitable rhBMP 2 slow releasing carriers currently available.

关 键 词：RHBMP-2 人骨形成蛋白2 珊瑚 骨诱导 人工骨 

分 类 号：R68[医药卫生—骨科学] R318.17[医药卫生—外科学]