人TMJ滑膜细胞的生长特性观察  

The growth characteristic of human TMJ synovial membrane cells in vitro

作　　者：王为[1,2] 司徒镇强[1,2] 陈建元[1,2] 吴军正[1,2] 朱南洲 李国华[1,2] 张纯妍[1,2] 刘忠 

机构地区：[1]大连210医院口腔科 [2]第四军医大学口腔医学院

出　　处：《实用口腔医学杂志》1998年第2期134-136,共3页Journal of Practical Stomatology

摘　　要：目的：研究体外培养人ＴＭＪ滑膜细胞生长特性和遗传稳定性。方法：采用ＭＴＴ法、ＨＥ染色及培养细胞染色体制备法，测定３种细胞（ＳＭＣ、ＴＧＣ、ＮＭＣ）的生长曲线、分裂指数、和贴壁率，观察不同代数的染色体数量和形态。结果：３种细胞体外生长都较为稳定，滑膜细胞生长速率略低于其它两种细胞，分裂指数最多为１５‰，贴壁率８ｈ为６０％，１２ｈ达９５％，不同代数的滑膜细胞染色体形态正常，数量均在２３对，遗传性状稳定。结论：体外培养人ＴＭＪ滑膜细胞生长稳定，在一定培养代数以内，生长较为规律，保持二倍体正常细胞特性，可以应用该细胞进行ＴＭＪ相关问题的研究。Objective:To investigate the growth characteristic of TMJ synovial membrane cells (SMCs) in vitro .Methods:The growth curve,population doubling time,attachment efficiency,cell division rate and the karyotype were investigated for SMCs,as well as TGCs and NMCs. Results:All the cells grew stably in vitro .SMCs were easier to be trypsinized than TGC, NMC and grew slower.The highest cell division rate of SMCs was 15‰.The attachment efficiency of SMCs was 60% in 8 hours, 95% in 12 hours respectively.Chromosomes analysis showed that more than 85% of SMCs had diploid karyotype with 23 pair of chromosomes. Conclusion:SMCs grow stably and keep diploid karyotype at least in 20 passages in culture.

关 键 词：颞下颌关节 滑膜细胞 体外培养 生长特性 

分 类 号：R782.6[医药卫生—口腔医学] R329－33[医药卫生—临床医学]