应用细胞因子单克隆抗体对人TMJ滑膜细胞的免疫组织化学研究  

Cytokine expression of SMCs and its regulation by IL 1

作　　者：王为[1,2] 陈建元[1,2] 司徒镇强[1,2] 吴军正 李焰[1,2] 朱南洲 李国华[1,2] 田杰[1,2] 

机构地区：[1]大连210医院口腔科 [2]第四军医大学口腔医学院

出　　处：《实用口腔医学杂志》1998年第2期141-143,共3页Journal of Practical Stomatology

摘　　要：目的：了解人ＴＭＪ滑膜细胞正常状态下参与细胞因子合成能力及ＩＬ－１对这方面的影响和调控作用。方法：采用体外培养人ＴＭＪ滑膜细胞（ＳＭＣ）及４种细胞因子和抗ＩＬ－２ｒ抗体，以ＡＢＣ法，对正常状态下ＳＭＣ的培养细胞和经ＩＬ－１作用４ｄ后的细胞进行免疫组织化学染色，观察细胞浆、膜上阳性物质分布情况。结果：正常状态ＳＭＣ对ＩＬ－１、ＩＬ－２ｒ（白介素２受体）、ＩＬ－６、ＴＮＦ表达均呈阳性或弱阳性，主要分布在胞浆内，但经ＩＬ－１作用后，几种抗体染色的阳性细胞比例大幅增加，阳性颗粒密度也增加，整个视野内增加一倍以上。结论：正常状态下，ＳＭＣ就有少量细胞参加ＩＬ－１、ＩＬ－６、ＴＮＦ的合成。Objective:To investigate the expression of IL 1 IL 2r IL 6 and TNF of TMJ synovial membrane cells (SMCs) and its regulation by IL 1.Methods:IL 1 IL 2r IL 6 and TNF expression of SMCs was examined with ABC immunohistochemistry method after the cells had been treated with IL 1 for 4 days. Results:Positive reaction to IL 1,IL 2r,IL 6 and TNF antibody was observed in some of SMCs in the control group.More and stronger IL 1,IL 2r,IL 6 and TNF expression was observed after the cells had been treated with IL 1 for 4 days.Conclusion:In normal condition a few SMC produce IL 1 IL 6 and TNF.IL 1 may be a synthesis inducer for other cytokines.

关 键 词：颞下颌关节 滑膜细胞培养 单克隆抗体 细胞因子 

分 类 号：R782.6[医药卫生—口腔医学]