颌面部肿瘤细胞冷冻的时间效应观察  

The effects of freeying on oral cancer cells

作　　者：王为[1,2] 司徒镇强[1,2] 赵重洋 朱南洲[1,2] 陈建元 毛天球[1,2] 李国华[1,2] 

机构地区：[1]大连210医院口腔科 [2]第四军医大学口腔医学院

出　　处：《实用口腔医学杂志》1998年第2期144-146,共3页Journal of Practical Stomatology

摘　　要：目的：研究冻融后癌细胞不同时间的生存率改变，建立准确测定冷冻对癌细胞损伤效应的方法。方法：采用体外培养的６种口腔癌细胞和两种正常细胞进行冷冻处理，ＭＴＴ法测定不同温度的生存率，选择－４０℃为敏感温度进行冻融后不同时间的冷冻后生存率测定。结果：不同温度冷冻癌细胞后测定结果显示：０℃～－３０℃细胞无明显损伤，－４０℃～－５０℃死亡率明显增加，达到４０％～６０％、－５０℃～－９０℃细胞死亡率未见明显增加。－４０℃冷冻后细胞死亡率随时间延长而有所增加，８ｈ达６０％～８５％，２４ｈ达８４％～９４％．结论：冷冻对癌细胞有明确杀伤作用，敏感温度在－４０℃～－５０℃。冻融后部分存活细胞的损伤无法修复，细胞死亡率随时间延长而缓慢增加，２４ｈ达到最大值。Objective: To study the effects of freezing temperature and time lapse after freezing on oral cancer cells.Methods:The cells of sixhuman oral cancer cell lines and two fibroblast cell lines were treated at 37 ℃～-80 ℃ for 1 min at each temperature point and thawed,then the survival rate of the cells was tested with MTT assay. The time dependancy of the cell death was studied at the strongest and heighest killing freezing temperature 0～24 hours after freezing.Resuts:More than 80% of survival was observed after the cells had been treated at 37 ℃～-30 ℃.More than 50% cells were killed by -40 ℃～-50 ℃ freezing.The survival fraction of the cells after -50 ℃～ -90 ℃ freezing was not significantly different from that after -40 ℃.The dead fraction of the cells increased in a time dependant manner in 24 hours following freezing and thaw.Conclusion:Freezing may kill oral cancer cells,the most effective and heighest freezing temperature may be -40 ℃～ -50 ℃;the death of the cells is time dependant in 24 hours after freez thaw.

关 键 词：颌面部肿瘤 癌细胞 冷冻 时间效应 

分 类 号：R739.81[医药卫生—肿瘤]