西藏绿萝花提取物对α-葡萄糖苷酶的抑制及抗氧化作用  

Study on Inhibitive Effect of Extracts from Scindapsus aureus on α-glucosidase and Its Antioxidant Activity

作　　者：竺琴[1] 张焜[1] 杜志云[1] 徐学涛[1] 蒋永福[1] 俞新华[2] 

机构地区：[1]广东工业大学轻工化工学院,广东广州510006 [2]华南农业大学生命科学学院,广东广州510642

出　　处：《中药材》2009年第1期89-92,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(20376015,20672023)

摘　　要：目的:研究西藏绿萝花提取物对α-葡萄糖苷酶的抑制及抗氧化作用。方法:将西藏绿萝花的70%乙醇提取物依次用石油醚、乙酸乙酯、正丁醇萃取,然后测定各部分对α-葡萄糖苷酶的抑制及抗氧化作用。结果:乙酸乙酯的萃取部分对α-葡萄糖苷酶的活性抑制较强,半数抑制浓度IC50为43.63μg/mL,比阿卡波糖抑制α-葡萄糖苷酶的IC50值250μg/mL低的多,抵制动力学表明为竞争性抑制,Ki值为21.49μg/mL;乙酸乙酯及正丁醇萃取部分具有较强抗氧化作用,0.42μg/mL的乙酸乙酯及正丁醇萃取部分对DPPH.自由基的清除率分别可达到88.5%、93.6%,与0.3 mg/mL的抗坏血酸对DPPH.自由基的清除率96.3%相当;乙酸乙酯萃取部分对氧自由基亦有一定的清除作用,IC50为0.27 mg/mL。结论:西藏绿萝花醇提取物的乙酸乙酯萃取部分有较强的α-葡萄糖苷酶抑制及抗氧化作用;正丁醇萃取部分有较强的抗氧化作用。Objective ： To study the inhibitive effect of extracts from Scindapsus aureus on α-glucosidase and its antioxidant activity. Methods： The 70% ethanol extracts was extracted by petroleum, ethyl acetate and n-butanol, and the inhibitory activity against α- glucosidases and antioxidative effects of each fraction were determined in vitro, and the inhibitory kinetics of ethyl acetate fraction was investigated. Results： The inhibitory activity of ethly acetate fraction against α-glucosidase was higher as its IC5o was 43.63 μg/mL The inhibition kinetics analyzed by Hnewaver-Burk plots showed that ethyl acetate fraction was a competitive type inhibitor, and the inhibition constants for free ennzyme （Ki） was determined to be 21.49μg/mL The antioxidative effects of ethly acetate and n-butanol fractions exhibited stronger than that of other fractions, the scavenging ability on DPPH · at the concentration of 0. 42 mg/mL were 88. 5% and 93.6%. Ethly acetate fraction showed scavenging ability on autioxidantor, and the ICs0 values was O. 27 mg/mL Conclusion： Ethyld acetate fraction of extract from Scindapsus aureus has potential inbibitroy activity against α-gtucosidase and antioxidant effects, and n-butanol fraction has great antioxidative effects.

关 键 词：绿萝花 Α-葡萄糖苷酶 抗氧化作用 

分 类 号：R285.5[医药卫生—中药学]