小剂量奥沙利铂抗肿瘤血管生成作用的体内实验研究  

An Experimental Study of Low Dose Oxaliplatin Inhibiting Tumor Angiogenesis in Vivo

作　　者：孙永臣[1] 钱晓萍[1] 刘宝瑞[1] 胡静[1] 胡文静[1] 禹立霞[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肿瘤中心,江苏南京210008

出　　处：《实用临床医药杂志》2009年第2期33-36,43,共5页Journal of Clinical Medicine in Practice

基　　金：江苏省自然科学基金资助项目(BK2006005)

摘　　要：目的观察小剂量奥沙利铂(L-OHP)对小鼠移植瘤生长的抑制作用及对血管内皮生长因子(VEGF)的表达和微血管密度(MVD)的影响。方法建立肝癌H22小鼠模型后,将小鼠随机分组4组,5%葡萄糖20 mL/kg阴性对照组、环磷酰胺20 mg/kg阳性对照组、奥沙利铂0.75 mg/kg和1.5 mg/kg组,腹腔等容积注射给药,连续10 d,第11天处死小鼠,计算瘤重抑制率,用免疫组化方法测定肿瘤组织中的VEGF和CD34的表达。结果1.5 mg/kg奥沙利铂组的瘤重明显低于阴性对照组(P<0.01),而且肿瘤组织中的VEGF和CD34的表达也明显低于阴性对照组(P<0.01)。结论小剂量奥沙利铂对H22肝癌荷瘤小鼠抑瘤作用明显,能抑制肿瘤组织中VEGF和MVD的表达;小剂量奥沙利铂在体内可能具有抑制肿瘤组织血管生成的作用。Objective To observe inhibition effect of low dose oxaliplatin（L-OHP） on the growth of tumor transplanted froma mouse and its effect on the expression of vascular endothecial growth factor （VEGF） and microvessel density（MVD）. Methods A mouse tumor model was set up by implanting H22 mouse liver cancer cells under the skins of mice. Forty experimental mice were divided randomly into four groups, including control 5 % glucose injection negative control group, cyclophosphamide（CTX） 20 mg/kg positive control group, Oxaliplatin 0.75 mg/kg group and Oxaliplatin 1.5 mg/kg group. The four groups were injected intraperitoneally with the same amount of injection, once a day for ten days. On Day 11 the mice were killed and the tumors were taken out and the tumor-weight-inhibition ratios were calculated. Tumors were examined by using immunohistochemical staining to study the expression of VEGF and MVD. Results The tumor- weight in the experimental oxaliplatin 1.5 mg/kg group was significantly lower than that of the negative control group（P〈0.01）, and so was the expression of VEGF and MVD（P〈0.01）. Conclusion Low dose oxaliplatin has significant inhibitory effect on the growth of the tumors and the expression of the VEGF and MVD in H22 liver cancer tumor-bearing mice; Low dose oxaliplatin maybe has inhibitory effect on angiogenesis of tumor tissue in vivo.

关 键 词：奥沙利铂 肿瘤 抗血管生成 VEGF MVD 

分 类 号：R73-3[医药卫生—肿瘤]