黄芩有效成分提取及对炎症反应的影响  

Separation of Scutellaria baicalensis and its anti-inflammatory activity

作　　者：朱伟[1] 张守勤[2] 孙红光[3] 朱迅[3] 

机构地区：[1]海南大学海洋学院制药工程系海南省热带水生生物技术重点实验室,海口570228 [2]吉林大学生物与农业工程学院,长春130025 [3]吉林大学基础医学院免疫教研室,长春130021

出　　处：《生物加工过程》2009年第1期69-73,共5页Chinese Journal of Bioprocess Engineering

基　　金：国家自然科学基金资助项目(30371334);海南省自然科学基金资助项目(80413);海南大学自然科学基金资助项目(kyjj0430)

摘　　要：利用常温超高压方法提取黄芩中黄酮类物质，得到黄芩甲醇提取物（SBM）。对该物质抗炎作用研究发现SBM（31．25-500mg／L）可抑制刀豆蛋白A（ConA）诱导的小鼠脾淋巴细胞增殖和脂多糖（LPS）诱导的腹腔巨噬细胞TNFα合成，SBM（125mg／L）的抑制率分别可达96％、99％；SBM（10mg／kg、20mg／kg）也可抑制大鼠棉球性肉芽肿，但不影响IKBα蛋白磷酸化。常温超高压方法可有效提取黄芩中黄酮类物质，黄芩提取物SBM可通过抑制淋巴细胞功能、炎症介质发挥抗炎作用。The extract-SBM was isolated from Scutellariae radix by high hydrostatic pressure. The effects of SBM on ConA-induced splenocyte proliferation, LPS-induced TNFα production, and protein phosphorylation of IκBα were studied in vitro, and the therapeutic effects of SBM on cotton pellet-induced rat granuloma was also investigated. SBM （31.25-500 mg/L） inhibited ConA-induced splenocyte proliferation in mice. SBM （31.25-250 rag/L） inhibited LPS-induced TNFα production. Cotton pellet-induced rat granuloma was significantly decreased by SBM with the doses of 10 mg/kg and 20 mg/kg. SBM could inhibit inflammatory reaction. Its mechanisms were related to the suppression of inflammatory medium production. High hydrostatic pressure treatment could been used to separate the medical plant.

关 键 词：炎症 IΚBΑ 黄芩 TNFΑ 

分 类 号：R284.2[医药卫生—中药学] R285.5[医药卫生—中医学]