孕哺期母鼠镧暴露对子代中枢神经系统的影响  

Effects of Lanthanum Chloride on Glutamate Concentration of Central Nervous System in Rats

作　　者：姜杰[1,2] 靳翠红[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院,辽宁沈阳110001 [2]辽宁省大连市疾病预防控制中心,辽宁大连116021

出　　处：《预防医学论坛》2009年第2期154-156,共3页Preventive Medicine Tribune

基　　金：辽宁省科学技术计划项目(项目编号:2005225008-3)

摘　　要：[目的]探讨氯化镧(LaCL3)对大鼠中枢神经系统谷氨酸能神经元的影响,以探讨LaCL3对神经系统的毒作用机制。[方法]成年雌性Wister大鼠受孕后用LaCL3染毒,实验组分别饮用La3+浓度为2.5、5.0和10.0 g/L的水溶液,对照组饮用蒸馏水。仔鼠断乳后用体积分数为10%的中性缓冲甲醛灌流处理并利用免疫组织化学方法和显微图像分析技术,分析大鼠脑组织切片中谷氨酸反应阳性细胞的阳性面积比、平均积分吸光度。[结果]0.5%LaCL3和1.0%LaCL3镧暴露组镧暴露子代大鼠大脑皮层、海马CA1、CA3区阳性面积比、平均积分吸光度与对照组相比明显升高,差异有统计学意义(P<0.05)。其中1.0%LaCL3镧暴露组平均积分光密度值与对照组比有显著差异(P<0.01)。[结论]孕哺期母鼠镧暴露后子代中枢神经系统中谷氨酸阳性面积比和平均积分光密度值均显著升高。[Objective]This study was conducted to explore the effects of Lanthanum Chloride （LaCL3） on glutamatergic neurons in rat central nervous system. [Methods]Groups of male Wistar rats were exposed with 0,2.5,5. 0, 10.0 g/L LaCL3. Distilled water was given to the controls. The percentage of positive area and integral optical density of glutamate reactive cells in rats CNS were evaluated by immunohistochemical method at the other day after the rats were decapitated and perfused with 10% bufferformaldehyde solution through left ventricle. [Results]The percentage of positive area and integral optical density of glutamate immunore active cell increased significantly （ P 〈20.05） in 0.5 % LaCL3 and 1.0 % LaCL3 comparison with control in cerebral cortex, hippocampus and cerebellum, and the activity of IA in 1.0% LaCL3 group increased significantly. [Conclusion]It is suggested that glutamate is likely to play a role in the excitatory neurotoxicity of LaCL3.

关 键 词：氯化镧 谷氨酸 中枢神经系统 神经毒性 

分 类 号：R74[医药卫生—神经病学与精神病学]