复合酶法提取桑叶中多糖的工艺条件优化  

Optimization Techniques for the Extraction of Polysaccharides from Mulberry Leaves by Complex Phosphoesterasum

作　　者：夏平[1] 谢何青[1] 

机构地区：[1]湖州师范学院生命科学学院,浙江湖州313000

出　　处：《安徽农业科学》2009年第1期198-199,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]采用复合酶法提取桑叶中多糖。[方法]通过单因素试验和正交试验研究了酶的浓度、酶作用的时间、酶作用的温度以及酶作用的pH值对桑叶粗多糖提取率的影响。[结果]通过复合酶法提高了桑叶多糖的提取率。[结论]提取的最佳工艺条件为:温度50℃、pH值为4.5、酶用量1.0%、提取时间1 h;提取桑叶多糖的收率可达14.32%。[Objective] The Complex Phosphoesterasum was used in the experiment to extract polysaccharide from mulberry leaves.[Method] Single factor tests and orthogonal experiment design methods were applied to analyze the influence of the factors such as the concentration,time,temperature and pH value of the enzyme on the extraction ratio of polysaccharides from mulberry leaves.[Results] The adoption of new extraction methods improved the extraction ratio of the target products.[Conclusion] The best conditions are as follows： temperature is 50 ℃,pH value is 4.5,the concentration of cellulose enzyme is 1.0% and the extracting time is 1 h.The extraction ratio of Polysaccharides in mulberry leaves is up to 14.32%.

关 键 词：复合酶 提取 桑叶 多糖 正交设计 

分 类 号：S888[农业科学—特种经济动物饲养]