拟南芥Ran小GTP结合蛋白在细胞有丝分裂中的定位  

The Localization of Arabidopsis thaliana Ran Small GTP-binding Protein in Cell Mitosis

作　　者：马立安[1,2] 江涛[3] 张忠明[2] 

机构地区：[1]长江大学生命科学学院,荆州434025 [2]华中农业大学农业微生物学国家重点实验室,武汉430070 [3]长江大学动物科学学院,荆州434025

出　　处：《华中农业大学学报》2008年第6期701-704,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30070370);美国国家科学基金项目(NSF-MCB0548525)资助

摘　　要：分别采用RT-PCR和直接荧光免疫方法分析Ran2 mRNA在拟南芥不同组织器官中的表达及Ran2的亚细胞定位。结果表明,Ran2基因在拟南芥根、茎、叶和花中均有表达;Ran2蛋白在细胞分裂间期主要定位于核膜周边,在后期定位于赤道板上和纺锤体上,末期又回到子细胞核膜周边。The expressionof .Arabidopsis Ran2 mRNA were analysed in different tissues by RTPCR, and the direct immunofluorescence microscopy was used to determine the subcellular localization of plant Ran proteins during the cell cycle. The results showed that Ran2 is expressed ubiquitously in Arabidopsis roots, stems, leaves and flowers. Ran2 protein was mainly localized at the nuclear envelope during interphase,at the equatorial plate and spindle during anaphase and at the surface of the daughter nuclei during telophase.

关 键 词：拟南芥Ran2小GTP结合蛋白 FITC-anti-Ran2抗体 亚细胞定位 

分 类 号：Q7[生物学—分子生物学]