α-连接素在胃癌转移淋巴结中再表达机制的研究  

Study on the mechanism of re-expression of α-catenin in lymph nodes of metastatic advanced gastric cancer

作　　者：刘津[1] 宋子军[1] 张国建[1] 薛平[1] 段国强[1] 赵琪[1] 李晓宁[1] 王凤安[1] 

机构地区：[1]河北医科大学第二医院胃肠外科,石家庄市050000

出　　处：《河北医药》2009年第1期12-14,共3页Hebei Medical Journal

摘　　要：目的探讨α-连接素（α-catenin）在进展期胃癌转移淋巴结内癌细胞的再表达情况及其临床意义。方法采用免疫组化、蛋白印记等方法检测30例胃癌患者胃癌原发灶和转移淋巴结中α-连接素蛋白表达情况。结果在30例胃癌原发灶中100%（30/30）α-连接素表达程度减低,胃癌转移淋巴结中50%（15/30）再表达阳性,其中高分化腺癌的再表达阳性率为71.42%（10/14）;中低分化腺癌的再表达阳性率为31.25%（5/16）。高分化腺癌相对于中低分化腺癌更易出现α-连接素的再表达阳性,两者比较差异具有统计学意义（P〈0.05）。30例原发肿瘤组织及相应转移淋巴结均检测到不同程度的α-连接素蛋白表达。原发癌组织中的各例蛋白相对强度为11～170,平均105±42,转移癌淋巴结组织中各例蛋白相对强度为8～112,平均40±28,原发癌组织中α-连接素蛋白表达相对强度高于转移淋巴结组织。在转移淋巴结中高分化腺癌蛋白表达相对强度为25～112,平均54±33,中低分化腺癌的蛋白表达相对强度为8～55,平均27±12。两者间蛋白表达相对强度差异具有统计学意义（P〈0.01）。结论胃癌转移淋巴结内癌细胞是存在α-连接素的再表达的,其蛋白再表达水平与肿瘤分化程度相关,并且对癌细胞在转移灶内聚集再生长起着重要作用。Objective To investigate the significance of re-expression of α-catenin in lymph nodes of advanced gastric cancer metastases. Me, otis The expression of α-catenin was detected in 30 patients with gastric cancer by immunohistochemistry and protein imprinting. Results The expression of α-catenin in primary focus of 30 patients was decreased（ 100% ）, while which was positive（50% ） in metastatic advanced gastric cancer, in which the positive rate in well-differentiated adenocarcinoma was 71.42% （10/14）, which was 31. 25% （5/16） in poorly differentiated adenocarcinoma. Compared with poorly differentiated adenocarcinoma, the well-differentiated adenocarcinoma was apt to positive of α-catenin expression, there was a significant difference bewteen the two different differentiated adenocarcinoma （ P 〈 0.05）. The positive expression of α-catenin was found both in primary tumor tissue and corresponding metastatic lymph nodes. The relative intensity of protein in primary carcinoma was 11 - 170 （mean 105±42）, which in lymph nodes of metastatic cancer was 8 - 112 （40±28）, the relative intensity of expression of α-catenin in primary carcinoma was higher than that of metastatic lymph node. The relative intensity of expression of α-cateninIn in well-differentiated adenocareinoma of metastatic lymph nodes was 25 - 112 （mean 54±33）, which was 8 - 55 （mean 27 ± 12） in poorly differentiated adenocarcinoma. There was a significant difference in relative intensity of expression of α-eatenin between the two different differentiated adenocarcinoma. Conclusion The re-expression of α-catenin exists in lymph nodes of metastatic advanced gastrie cancer, and the re-expresian levels are related with the extent of tumor differentiation, furthermore, which plays an important role in gathering and growth of cancer cells in metastatic focus.

关 键 词：胃癌 淋巴转移 免疫组化 蛋白印记 再表达 

分 类 号：R735.2[医药卫生—肿瘤]