脑胶质瘤中p33ING1蛋白表达及其与细胞凋亡和增殖关系的研究  

Study on the relationship between the expression of p33ING1 gene and tumor cell proliferation as well as apoptosis in human gliomas

作　　者：姚志刚[1] 焦保华[2] 戴缤[3] 孙晓立[1] 

机构地区：[1]石家庄市第三医院神经外科,河北省050011 [2]河北医科大学第二医院神经外科 [3]北京市世纪坛医院神经外科

出　　处：《河北医药》2009年第2期151-153,共3页Hebei Medical Journal

摘　　要：目的检测p33 ING1蛋白在脑胶质瘤中的表达,探讨其与肿瘤细胞增殖、凋亡及肿瘤恶性程度的关系。方法应用免疫组化S-P法分别检测p33 ING1和增殖细胞核抗原(PCNA)的表达,并用TUNEL法做原位细胞凋亡检测,实验数据进行统计分析。结果50例脑胶质瘤中p33ING1蛋白表达的总阳性率为90%(28/50),随着胶质瘤恶性度的增高其表达水平下降,而且表达水平与细胞凋亡指数(AI)呈正相关与细胞增殖指数(PI)呈负相关(P<0.01)。结论胶质瘤细胞中抑癌基因p33ING1的表达可促进细胞凋亡,抑制细胞过度增殖,与胶质瘤恶性程度密切相关。检测其表达水平的变化对胶质瘤的诊断、治疗及判断预后均有重要指导意义。Objective To investigate the relationship between the expression of p33ING1 gene and tumor cell proliferation, apoptosis, malignant degree in human gliomas. Methods Apoptotic cells and apoptotic bodies were detected by TdT-mediated dUTP-biotin nick -end labeling （TUNEL） method S-P stains were conducted to examine the expression of p33ING1 and PCNA. Results The percentage of p33ING1 positive expression was 90% in 50 specimens, which was decreased with the increase of malignant degree of glioma. Its expression levels were related positively to apoptnsis index（M, P 〈 0.01 ）, however, which were related negatively to cell proliferation index（P I, P 〈 0. 01 ）. Conclusion Expression of suppressor gene-p33ING1 can promote apoptosis of glioma cells and inhibit cell proliferation, which may play an important role in the development and malignant progression of gliomas. To detecte expression of p33ING1 has an important instructional significance in early diagnosis, gene interventional therapy and prognostic judgement for patients with glioma.

关 键 词：胶质瘤 P33 ING1 凋亡 增殖 

分 类 号：R739.41[医药卫生—肿瘤]