膀胱癌基底膜层粘蛋白、胶原蛋白Ⅳ与纤维连接蛋白分布表达的相关性研究  

Relative study on the relationship among laminin、collagen type Ⅳ and fibronectin distribution and expression at basement membrane in bladder carcinoma

作　　者：沈周俊[1] 陈杭 丁伟[2] 史时芳[1] 张志根[1] 陈昭典[1] 朱选文[1] 沈志坚[1] 叶慧慧[2] 余心如[2] 李炜 

机构地区：[1]浙江医科大学附属第一医院泌尿外科 [2]杭州浙江医科大学附属第一医院泌病理科,310003 [3]杭州浙江医科大学附属第一医院泌进修医师,310003

出　　处：《中华实验外科杂志》1998年第1期46-47,共2页Chinese Journal of Experimental Surgery

摘　　要：目的探讨膀胱癌基底膜中层粘蛋白、胶原蛋白Ⅳ与纤维连接蛋白分布表达的相关性。方法用免疫组织化学方法测定35例膀胱移行上皮癌和3例低分化鳞状上皮癌肿瘤组织基底膜层粘蛋白(LN)、胶原蛋白Ⅳ(Col Ⅳ)和纤维连接蛋白(FN)含量与分布。结果病理级别高、浸润性生长的膀胱移行上皮癌,基底膜LN、Col Ⅳ和FN一样,阳性染色少,分布不连续;反之,病理级别低、非浸润性生长的肿瘤,基底膜LN、Col Ⅳ也和FN一致,阳性染色多,分布呈连续性。低分化鳞状上皮癌基底膜LN、Col Ⅳ和FN分布含量同样呈正相关。结论膀胱肿瘤基底膜LN、Col Ⅳ和FN分布含量高度一致,它们的丧失可作为肿瘤恶性度高的指标之一。Objective In order to explore the relationship among laminin (LN), collagen type Ⅳ (Col Ⅳ) and fibronectin (FN) express on at basement membrane (BM) in patients with bladder carcinoma and their clinical implications. Methods Distribution of LN, Col Ⅳ and FN at BM were studied by immunoperoxi- dase technique in 35 patients with bladder transitional cell carcinoma and 3 with squamous cell carcino- ma. Results The poorer the tumour differentiation or the deeper the tumour invasion, the more the loss of LN, Col Ⅳ and FN at BM in patients with bladder transitional cell carcinoma. The expressions of LN, Col Ⅳ and FN at BM in patients with squamous cell carcinoma were reduced. Conclusions The losing de- grees of LN and Col Ⅳ corresponded to the losing degree of FN. The absence of LN, Col Ⅳ as well as FN at BM can be used to predict the malignant tendency in patients with bladder neoplasm.

关 键 词：膀胱肿瘤 层粘蛋白 胶原蛋白Ⅳ 纤维连接蛋白 

分 类 号：R737.140.2[医药卫生—肿瘤]