脉络膜新生血管动物模型的建立与评估  

Establishment and quantitative analysis of animal model of choroidal neovasculari-zation

作　　者：李学晶[1] 唐由之[1] 王慧娟[1] 冯俊[1] 张励[1] 

机构地区：[1]中国中医科学院眼科医院,中国北京市100040

出　　处：《国际眼科杂志》2009年第2期246-249,共4页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30472229)~~

摘　　要：目的：评价氪激光诱导棕色挪威（brown norwav，BN）大鼠脉络膜新生血管（choroidal neovascularization，CNV）模型的可行性。方法：BN大鼠32只一眼行氪激光（659nm）眼底光凝，另一眼作空白对照。激光功率、光凝斑直径和曝光时间分别为360mW、50μm及0．05出，分别大光凝后7，14，21,28d随机选取7只大鼠行眼底照像、荧光素眼底血管造影（FFA）检查。随机选取1只大鼠行脉络膜血管铺片检食。眼球标本行组织病理切片光镜、免疫组织化学染色观察。结果：光凝后7d，CNV开始形成，21d达到高峰，14～28d CNV无明显变化。7～21d，FFA显示造影晚期荧光素渗漏及光镜下CNV厚度逐渐增加，28d时可能因瘢痕开始形成，故FFA渗漏和CNV厚度开始减少。结论：氪激光诱导BN大鼠的CNV模型是一种较为理想的模型，FFA及CNV厚度分析是CNV定虽研究的有效手段。AIM： To investigate the feasibility of Krypton laser-induced choroidal neovasculation （CNV） model in the Brown Norway rats. METHODS： One eye of 32 rats received a series of 20 spots of laser irradiation （659nm, 360mW, 50μm, 0.05s）, the other eye were as controls. CNV was evaluated by fundus fluorescein angiography （FFA）, high molecular weight FITC-Dextran （MW 2 × 106） for high resolution angiography in RPE-choroid-sclera flat mounts, and histopathologic examination was performed in 7, 14, 21 and 28 days after photocoagulation. RESULTS. CNV was firstly appeared on day 7 after photocoagulation, reaching the peak on day 21, no significant progress occurred in 14-28 days. The fluores- cein leakage and the thickness of laser-induced CNV were increased from day 7 to day 21, were decreased in day 28. CONCLUSION. The Krypton laser induced model of CNV in the pigment rats may be useful, and the quantitative analysis of FFA and the thickness of CNV are useful for in vivo studies of angiogenesis and its modulation via various therapy.

关 键 词：氪激光 脉络膜新生血管 动物模型 

分 类 号：R773.4[医药卫生—眼科] R-332[医药卫生—临床医学]