兔眼玻璃体腔内注射透明质酸酶诱导形成玻璃体后脱离  

Posterior vitreous detachment induced by intravitreal injection of hyaluronidase in the rabbit

作　　者：朱格非 秦波 席兴华 周秀珍 曾婷 

机构地区：[1]暨南大学第二临床学院深圳市人民医院眼科,中国广东省深圳市518020

出　　处：《国际眼科杂志》2009年第2期254-256,共3页International Eye Science

基　　金：中国广东省深圳市科技计划项目资助(No.200603164)~~

摘　　要：目的:考察透明质酸酶(hyaluronidase,HS)兔眼内注药诱导形成玻璃体后脱离(posterior vitreous detechment,PVD)的作用。方法:取新西兰大白兔20只,将HS5U注入20只右眼玻璃体腔内,左眼内各注入等量生理盐水作为对照。在设定的时间点(15min^7d),通过活体的直、间接眼底镜、视网膜电图(electroretinogram,ERG)检查和摘除眼球的光、电镜检查,从形态学方面对酶作用于玻璃体的效应及其眼部毒性进行观测。结果:在实验观察期内,实验眼从第3d开始有肉眼可见的PVD形成;所有眼ERGb波振幅(bA)注药前后比值没有明显差异;光、电镜下视网膜的各层结构在玻璃体注射后无明显改变。结论:玻璃体腔内注射5UHS酶可有效地诱导产生PVD,而且对视网膜的结构和功能无毒性损害。AIM： To study the effect of pharmacologic induction of posterior vitreous detachment （PVD） by intravitreal injection of hyaluronidase （HS）. METHODS： 5U of HS was injected into the vitreous cavity of twenty right eyes of New Zealand white rabbits. The left eyes, intravitreally injected by same volume of NS, were considered as control. Observation period was from 15 minutes to 7 days. Ophthalmoscopy, electroretino- graphy（ERG） and histological examination were performed to assay morphologically the enzymes＇s effec-tiveness to produce PVD and their probable toxicity to the retina. RESULTS： During the observation period, clinical PVD found by naked eye could be successfully induced in the right eyes on the third postinjection day; no statistical difference was found in the value of ERG＇b-wave amplitudes （bA） ratio; and histological examination showed normal structure of the retina in the treated eyes. CONCLUSION; Intravitreal injection of HS can induce PVD successfully, and is safe to the retina＇s structure and function of the rabbit eyes.

关 键 词：透明质酸酶 玻璃体后脱离 兔 

分 类 号：R776.4[医药卫生—眼科]