人翼状胬肉组织HMG-CoA还原酶和LDL受体基因的表达  

Expression of low-density lipoprotein receptors and hydroxy methylglutaryl-coenzyme A-reductase in human pterygium

作　　者：喻谦[1] 柳林[2] 王永志[3] 

机构地区：[1]成都医学院第一附属医院眼科,中国四川省成都市610050 [2]上海第二军医大学第一附属医院眼科,中国上海市200433 [3]上海第二军医大学微生物教研室,中国上海市200433

出　　处：《国际眼科杂志》2009年第2期284-285,共2页International Eye Science

摘　　要：目的:研究LDL-R基因和HMG-CoA-R基因在翼状胬肉组织中的表达。方法:随机选取原发性和复发性翼状胬肉组织各10例,正常结膜组织10眼。实时荧光定量PCR技术分析结膜中HMG-CoA-R和LDL-R的相对mRNA含量。结果:原发性和复发性翼状胬肉中的HMG-CoA-R及LDL-R的mRNA水平均明显高于正常球结膜组织,差异具有统计学意义(P<0.05)。结论:HMG-CoA-R和LDL-R基因可能参与翼状胬肉的发病过程。AIM： To quantitatively detect gene expression level of low-density lipoprotein receptors （LDL-R） and hydroxy methylglutaryl-coenzyme A-reductase （HMG-CoA-R） in human primary pterygium, reccurent pterygium and conjunctiva. METHODS： Primary pterygium, recurrent pterygium and normal conjunctiva samples were obtained from 30 eyes at the time of surgery during February 2007 to February 2008. Quantitative real-time PCR was used to analyze the content of HMG-CoA-R and LDL-R gene in pterygium and normal tissue. RESULTS： QRT-PCR revealed a statistically significant increase of LDL-R mRNA levels. 4. 1 fold in primary pterygia and 2.7 fold in recurrent pterygium, compared with the control normal conjunctiva. The differences had statistical significance （ P 〈 0.05）. Also the mRNA levels of HMG-CoA-R were increased significantly in both primary and recurrent pterygia by 4. 2 fold and 3. 6 fold respectively, compared with normal conjunctiva, with statistical significance （ P〈 0.05）. CONCLUSION. This study indicates that pterygium have an altered metabolism of cholesterol namely increased LDL-R and HMG-CoA-R mRNAs, which is characteristic of tumorlike tissues. Both genes may play important roles in the pathogenesis of pterygium.

关 键 词：翼状胬肉 低密度脂蛋白受体 羟甲基戊二酰辅酶A还原酶 实时荧光定量逆转录聚合酶链反应 基因表达 

分 类 号：R777.3[医药卫生—眼科]