具不同蛋白质含量水稻品种中天冬酰胺合成酶基因的表达  

Expression Characteristics of Asparagine Synthetase Gene among Rice Varieties with Different Seed Storage Protein Content

作　　者：周丽慧[1] 张昌泉[1] 刘巧泉[1] 顾铭洪[1] 

机构地区：[1]扬州大学农学院,教育部植物功能基因组学重点实验室,江苏省作物遗传生理重点实验室,扬州225009

出　　处：《基因组学与应用生物学》2009年第1期39-45,共7页Genomics and Applied Biology

基　　金：国家自然科学基金重点项目(30530470);国家863计划(2006AA10A102);教育部新世纪优秀人才支持计划项目(NCET-07-0736)资助

摘　　要：为研究水稻中氮同化与籽粒蛋白质合成可能的关系,本实验通过Northern杂交分析比较了氮同化关键酶—天冬酰胺合成酶基因(OsAS)在不同水稻品种中的表达特性。结果显示,OsAS基因在水稻不同组织及其不同发育时期中的表达水平不同,以发育种子中的表达量最高,叶鞘和叶片中相对较弱;在籽粒发育过程中OsAS基因的表达呈先升高后下降的趋势。具不同种子蛋白质含量水稻品种叶片中OsAS基因的表达水平存在显著差异,相关性分析显示在籼亚种或粳亚种不同品种内OsAS基因表达量与籽粒蛋白质含量可能存在一定的关联。In this study, the expression pattern of the OsA S gene encoding asparagine synthetase was studied via Northern blotting analysis, and the possible relationship between nitrogen assimilation and the content of seed storage proteins was analyzed. The results showed that the expression level of OsA S gene varied within different tissues and development periods, it preferred a highest expression level in spikelets, but very low in leaf sheath and blade. During grain developing, the OsA S transcript in spikelets increased firstly, and then declined rapidly. Moveover, the data indicated that the expression level of OsA S gene in the blade was siginificant different among different rice varieties, and there might exhibit a certain correlation between the OsA S expression level and seed protein content among different varieties of the same indica or japonica subspecies.

关 键 词：水稻 天冬酰胺合成酶基因 蛋白质含量 基因表达 

分 类 号：S511[农业科学—作物学]