φC31整合酶与转基因动物研制  

φC31 Integrase and Transgenic Animals Research

作　　者：曲立娟[1] 黄英[1] 

机构地区：[1]上海交通大学医学遗传研究所,上海200040

出　　处：《中国生物工程杂志》2009年第2期103-107,共5页China Biotechnology

基　　金：国家自然科学基金(30600331);国家"863"计划(2007AA100502)资助项目

摘　　要：链霉菌噬菌体φC31整合酶是一种位点性特异重组酶。它以单向整合方式进行重组,无须其他辅助因子,且整合效率高、表达稳定,所以近年来越来越多地被用来介导外源基因与宿主基因组的特异性整合,并被应用于哺乳动物的转基因整合技术中,它为攻克转基因动物研制过程中的随机整合、整合效率低、表达水平不高等技术瓶颈提供了新思路。对链霉菌噬菌体φC31整合酶的作用机制和特点优势作了简要的阐述,并对其广阔的应用前景作一展望。Streptomyces phage φC31 integrase is a site-specific recombinase and introduce foreign gene to unidirectional recombination. Because of its integration mediated by φC31 integrase without any cofactors and especially directed integration, it possesses high efficiency and stable expression of foreign gene. These advantages make it an attractive tool for genetic engineering. It has been widely used to transgene integration in mammalians and provides an opportunity to overcome the bottlenecks in the generation of transgenetic animals, such as random integration, low integral rate, low level of expression and so on. The φC31 integrase will have a wide application in the near future.

关 键 词：ΦC31整合酶 转基因动物 位点特异整合 

分 类 号：Q78[生物学—分子生物学] Q75