不同浓度的互隔交链孢霉素对NIH/3T3细胞cAMP水平的影响  

The Effect of Different Concentrations of Altenuene on cAMP Level of NIH/3T3 Cells

作　　者：王瑾瑾[1] 江亚南[1] 马艳英[1] 黄幼田[1] 郑智敏[1] 赵继敏[1] 董子明[1] 

机构地区：[1]郑州大学基础医学院病理生理教研室,河南郑州450052

出　　处：《肿瘤基础与临床》2009年第1期8-10,共3页journal of basic and clinical oncology

基　　金：国家自然科学基金(编号:30471952);河南省自然科学基金(编号:2007310020)

摘　　要：目的观察不同浓度的互隔交链孢霉素(altenuene,ALT)对小鼠胚胎成纤维细胞(NIH/3T3)中cAMP水平的影响。方法以NIH/3T3为研究对象,观察以0、2.5、5、10、15和20μmol/L ALT作用30min,并设0.4mmol/L烷化剂甲璜酸甲酯(methyl methane-sulfonate,MMS)为阳性参照物,使用超声法破碎细胞,以125I标记的放射免疫分析(RIA)检测细胞内cAMP水平。结果与对照组相比,5、10、15和20μmol/L ALT能升高细胞内cAMP水平(P<0.05);而以0.4mmol/L MMS处理细胞,cAMP水平同样升高。结论ALT对细胞具有明显的影响,可能与经由第二信使cAMP所介导的相关信号通路有关。Objective To observe the effects of Ahenuene of different concentrations on cAMP in NIH/3T3 cell line. Methods NIH/3T3 cells were cultured for 30 rain in the medium supplemented with ALT at 0, 2.5, 5, 10, 15, 20 μmol/L and MMS at 0.4 mmol/L for positive control. Then the level of cAMP in NIH/3T3 cell was observed by ^125I-cAMP RIA. Results After NIH/3T3 cells were exposed to different concentrations of ALT for 30 min, the cellular level of cAMP was significantly increased in a concentration-dependent, and in positive control group the level of cAMP increased the same （P 〈 0.05 ）. Conclusion ALT can increase the concentrations on cAMP in NIH/3T3 cells, which related to the single pathway via the second messenger cAMP possibly.

关 键 词：互隔交链孢霉素 环腺苷酸 NIH/3T3细胞 

分 类 号：R730.231[医药卫生—肿瘤]