淫羊藿黄酮对APP695基因转染神经细胞β-淀粉样肽及β-分泌酶的影响  

Effects of Epimedium Flavanoids on β-amyloid in APP695 cDNA-transfected Nerve Cells and on β-secretase Activity in Vitro

作　　者：杨潘[1] 宋秋洁[1,2] 王月华[3] 李雅莉[1] 杜冠华[3] 李林[1] 

机构地区：[1]首都医科大学宣武医院药物研究室,神经变性病教育部重点实验室,北京市100053 [2]国家食品药品监督管理局药品评价中心,北京市100045 [3]中国医学科学院药物研究所,北京市100050

出　　处：《中国康复理论与实践》2009年第2期126-128,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家重点基础研究计划-973计划项目(No.2003CB517104);国家自然科学基金项目(No.90709011;30472184)

摘　　要：目的观察淫羊藿黄酮(EF)对APP695基因转染SH-SY5Y细胞中β-淀粉样肽(Aβ)水平的影响。方法利用免疫沉淀+Western印迹法、放射免疫法分别检测细胞培养上清液和细胞裂解液中的Aβ水平。通过测定β-分泌酶(BACE1)切割荧光底物所得的荧光值检测BACE1活性。结果EF2.5～25g/ml与APP695基因转染SH-SY5Y细胞模型共孵育24h能明显降低模型细胞培养液中的Aβ含量(P<0.05,P<0.01)。体外试验中EF50g/ml对BACE1活性的抑制率为95.0%。结论EF能降低APP695基因转染细胞生成和分泌Aβ。Objective To investigate the effects of Epimedium flavanoids （EF） on β-amyloid （Aβ） in APP695 cDNA-transfected human neuroblastoma cell line SH-SYSY cells. Methods The immunoprecipitation plus Western blotting and radioimmunoassay were used to detect Aβ concentration in the supernatant of cell culture and cell disruption fluid. The activity of β-secretase （BACE1） was measured by detecting the fluorescence value of substrate cut by BACE1. Results Incubation of EF 2.5-25 g/ml with APP695 cDNA transfected SH SYSY cells for 24 h significantly decreased Aβ content in the supernatant of cell culture （ P d0.05, P d0.01）. EF 50 g/ml inhibited the activity of BACE1 by 95.0% in vitro. Conclusion EF can decrease Aβ production and secretion of APP695 cDNA-transfected nerve cells.

关 键 词：淫羊藿黄酮 Β-淀粉样肽 Β-分泌酶 阿尔茨海默病 

分 类 号：R749.1[医药卫生—神经病学与精神病学]