节杆菌10137产β-呋喃果糖苷酶的分离纯化的研究  

Separation and purification of β-fructofuranosidase from Arthrobacter 10137

作　　者：许牡丹[1] 姚甲玉[1] 王亚娟[1] 杨滋[1] 

机构地区：[1]陕西科技大学生命科学与工程学院,西安710021

出　　处：《食品科技》2009年第2期11-13,共3页Food Science and Technology

摘　　要：采用硫酸铵盐析、透析脱盐、丙酮沉淀、Sephadex G-75凝胶色谱等分离纯化技术,从节杆菌培养液分离纯化了β-呋喃果糖苷酶,纯化倍数为24.93,回收率为47.35%,经SDS-聚丙烯酰胺凝胶电泳后有一条明显的蛋白质谱带。The crude β-fructofuranosidase prepared from Arthrobacter sp. 10137 was fractioned in a sequence of operations including ammonium sulfate fraction, dialysis, acetone deposition and column chromatography with sephadex G-75 purification of about 24.93 fold was achieved with an overall yield of 47.35%. The pure enzyme showed a single protein band by SDS-PAGE.

关 键 词：节杆菌 Β-呋喃果糖苷酶 分离和纯化 

分 类 号：TS201.25[轻工技术与工程—食品科学]