连翘花水提物保护线粒体及抗衰老研究  

Protective effects on mitochondria and anti-aging activity of aqueous extract of forsythia suspensa flower

作　　者：李兴泰[1] 李洪成[1] 陈瑞[1] 

机构地区：[1]大连民族学院生命科学学院,大连116600

出　　处：《食品科技》2009年第2期220-224,共5页Food Science and Technology

基　　金：辽宁省教委高等学校科学研究基金资助项目(994421792)

摘　　要：目的:探索连翘花水提物(FFA)是否对自由基所致的线粒体损伤有保护作用,并研究其抗氧化能力、超氧阴离子的清除活性及抗衰老机制。方法:采用硫代巴比妥酸(TBA)比色法测定丙二醛(MDA)含量、分光光度法测线粒体的肿胀度,并以还原型辅酶I-氮蓝四唑-吩嗪硫酸甲酯(NADH-NBT-PMS)为超氧阴离子生成系统测定对超氧阴离子的清除能力。小鼠颈背部皮下注射D-半乳糖(D-gal)100 mg/kg.d,连续给药7周造衰老模型,利用钼酸胺比色法、黄嘌呤氧化酶法、二硫代二硝基苯甲酸比色法、Fenton反应显色法分别测定FFA对小鼠肝匀浆过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及抗羟自由基活力的影响。结果:FFA可明显抑制脑、肝匀浆及肝线粒体的脂质过氧化、线粒体肿胀,并呈剂量依赖关系,清除超氧阴离子的能力显著,且能明显增强CAT、SOD、GSH-Px及抗羟自由基活力。结论:FFA能通过清除活性氧自由基及提高抗氧化酶活力来保护线粒体,具有一定的抗氧化及抗衰老药用价值。Objective： To investigate whether aqueous extract of Forsythia suspensa flower（FFA） have protective effect on mitochondrial injury induced by free radicals, antioxidation and superoxide anion（O2^-） scavenging activities and antiaging mechanism. Methods： TBA colorimetric method was used to determine malondialdehyde （MDA）, spectrometric method to measure the swelling of mitochondria and activities of scavenging O2^- which was produced by reduced nicotinamide adenine dinucleotide（NADH）-4-Nitrobluetetrazolium chlodde（NBT）-N- MethylpHenazonium methyl sulfate（PMS） system. The neck back of mice were injected subcutaneously with D- galactose to induce aging model at a dose of 100 mg/kgod for 7 weeks. The effect of FFA on the activities of catlase（CAT）, surperoxide dismutase（SOD） and glutathione peroxidase（GSH-Px） and anti-hydroxyl radical were assayed by ammonium molybdate colorimetry, xanthine oxidase method, dithiodinitrobenzoic acid colorimetry and Fenton reaction colorimetry in vivo respectively. Results： FFA could inhibit brain,liver homogenate,liver mitochondrial lipid peroxidation and mitochondrial swelling induced by Fe^2＋-L-Cys in a concentration-dependent manner and also had significant O2^- scavenging effect. Moreover, the activities of CAT, SOD, GSH-Px and anti-hydroxyl radical in mice liver homogenate were increased significantly by FFA. Conclusion： FFA protect mitochondria by scavenging reactive oxygen species, and increasing the activity of antioxidase. So had the pharmaceutical values of antioxidant and anti-aging.

关 键 词：连翘花 活性氧 抗氧化酶 线粒体 抗衰老 

分 类 号：TS202.3[轻工技术与工程—食品科学]