NKX3.1基因沉默对LNCaP细胞基因表达谱的影响  

Effects of NKX3.1 gene knockdown on gene expression profile in LNCaP cells

作　　者：朱镭 倪国新[1] 张政希[1] 程培[1] 徐学敏[1] 林标扬[1] 李伟 

机构地区：[1]上海交通大学系统生物医学研究院,上海200240

出　　处：《山东医药》2009年第1期29-32,共4页Shandong Medical Journal

基　　金：上海市科委重点实验室专项基金资助项目(05dz22321)

摘　　要：目的探讨降低NKX3.1表达对雄激素依赖性前列腺癌细胞系(LNCaP)基因表达谱的影响。方法用表达NKX3.1 shRNA的质粒转染LNCaP细胞,经Northern blot和Western blot检测其干扰NKX3.1 mRNA和蛋白表达的效果,基因表达芯片法比较转染shRNA的细胞与转染空载体的对照细胞之间mRNA表达的差异。结果shRNA干扰可降低LNCaP细胞内NKX3.1蛋白的水平,可引起115种基因表达下调,114种基因表达上调;在严谨条件下进行功能聚类可得到与细胞凋亡相关、与糖代谢相关和与氨基酸代谢相关等共7个基因功能富集组。结论NKX3.1蛋白水平下调可引起LNCaP细胞基因表达谱的显著改变,其中一些基因表达的改变可能与NKX3.1的肿瘤调节活性密切相关。Objective To study the effects of decreasing NKX3.1 protein expression on gene expression profiles in the androgen-dependent LNCaP prostate cancer ceils. Methods Plasmids expressing shRNAs of NKX3. 1 gene were transfected into LNCaP cells, Nothern blot and Western blot analyses were conducted to confirm the efficiency of RNA interference on mRNA and protein, cDNA microarray were used to profile the changes of mRNA level between shRNA transfected cells and empty vector transfected control cells. Results Decreasing NKX3. 1 protein level by shRNA interference induced downregulation of 115 genes and upregulation of 114 genes. Nine groups of genes including apoptosis-related, carbohydrate and amino acid metabolism related genes were enriched by functional classification under stringent conditions. Conclusions Decreasing the NKX3. 1 protein level significantly altered the gene expression profiles in LNCaP cells. Some of these gene expression changes may be closely correlated with the tumor modulator activities of NKX3.1.

关 键 词：NKX3.1 SHRNA 前列腺肿瘤 基因表达谱 

分 类 号：R730.231[医药卫生—肿瘤]