产漆酶真菌筛选及其固态发酵条件的初步研究  

Screening of Laccase-Producing Fungal Strain and the Preliminary Study on the Solid-State Fermentation Conditions

作　　者：施晓燕[1] 蔡宇杰[1] 王志新[1] 廖祥儒[1] 张大兵[2] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江苏汉邦科技有限公司,江苏淮安223001

出　　处：《食品与药品》2009年第1期11-14,共4页Food and Drug

摘　　要：目的筛选产漆酶真菌,优化固态发酵培养基组成,研究粗酶的酶学性质。方法平板筛选获得菌株,通过测定漆酶活性优化培养基的组成。结果获得了固态发酵条件下产漆酶活性较高的菌株。优化后培养基的组成:豆粕和木屑比例1∶1,木糖0.5%,酒石酸铵2.0%,培养基含水量65%。漆酶最适温度60℃,低于50℃时漆酶较稳定,最适pH3.5,pH6.5～9.0之间漆酶稳定性较好。结论得到了产漆酶活性较高的菌株,培养基优化后漆酶活性提高7倍。Objective To Screen the laccase-producing fungi and optimize its solid-state fermentation medium as well as study the property of crude enzyme. Methods The strains were obtained by plate screening and the optimization of medium was performed by measuring the activity of laccase. Results A fungal strain with a high laccase-producing activity was obtained under solid-state fermentation. The optimal medium contained： soybean meal and sawdust with the ratio of 1 ： 1, 0.5 % xylose, 2.0 % ammonium tartrate and 65 % moisture. The optimal temperature was 60 ℃ for laccase, and the laccase was stable at less than 50 ℃ The optimal pH value was 3.5, and the laccase was stable at pH 6.5 - 9.0. Conclusion A fungal strain with a high laccase-producing activity was obtained and the activity of laccase was increased 7 times with the optimization of medium.

关 键 词：漆酶 筛选 固态发酵 粗酶性质 

分 类 号：Q554[生物学—生物化学]