曲古菌素A对去乙酰化酶2在子宫内膜癌细胞株HEC-1B中表达的影响  

Effect of trichostatin A on the expression of HDAC2 in HEC-1B cells.

作　　者：张璇[1] 李茗[1] 王泽华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院妇产科,武汉430022

出　　处：《华中医学杂志》2009年第1期24-27,共4页Central China Medical Journal

摘　　要：目的研究曲古菌素A(TSA)对子宫内膜癌细胞株HEC-1B中去乙酰化酶2(HDAC2)和p53表达的影响,及TSA对HEC-1B增殖的抑制作用,探讨TSA诱导HEC-1B细胞增殖抑制的机制。方法用不同浓度的TSA(100、200、400nmol/L)分组培养HEC-1B细胞(0、24、48、72h),用四甲基偶氮唑蓝(MTT)法测定不同浓度、不同时间的TSA作用后子宫内膜癌HEC-1B细胞的增殖率。应用Western Blot以及RT-PCR方法检测HDAC2和p53的表达水平,研究TSA对其表达的影响。结果TSA对HEC-1B细胞的增殖具有明显的抑制作用,并呈时间依赖性(P<0.01)。HDAC2在该细胞中的表达随TSA的剂量和时间呈减弱趋势,而p53的表达呈升高趋势。结论TSA具有去乙酰化酶抑制剂作用,抑制HDAC2的表达并促进肿瘤抑制因子p53的活化与表达,能够抑制子宫内膜癌细胞的增殖。Objective To investigate the depressant effect and mechanism of trichostatin A （TSA） on proliferation and being induced apoptosis of Histone deacetylase 2 （HDAC2） and p53 in human endometrial cancer-1B cell line （HEC-1B）. Methods Three levels concentration of TSA （ 100, 200,400 nmol/L） on HEC-1B at different time duration （0, 24,48, 72 hours） were applied. The expression of HDAC2 in HEC-IB was examined using Western Blot and the data was analyzed with different concentrations of TSA at various treating time duration. The proliferation and apoptotic rates of HEC-1B and the effect of TSA on them were determined using MTT method. At the same testing condition, the expression level of HDAC and p53 in HEC-1B and the effect of TSA were determined using reverse transeription-polymerase chain reaction （RT-PCR）. Results TSA has significant inhibition effect on proliferation of HEC-1B, which shows the trend of time duration dependency. With the increasing of TSA dose and time duration, expression of HDAC2 in cancer cells attenuated, but that of p53 increased. Conclusion TSA had the HDAC inhibitor＇s character, which could inhibit the expression of HDAC2 and promote activation and expression of p53, inhibit proliferation of HEC-1B at the same time.

关 键 词：曲古菌素A 去乙酰化酶2 P53蛋白 HEC-1B细胞 

分 类 号：R737.33[医药卫生—肿瘤]