IL-10对缺氧/复氧大鼠脑皮层神经元细胞内pH和游离Ca^(2+)的影响  

Effect of IL-10 on variation of intracellular pH and calcium ions in the cultured rat cortical neurons following hypoxic and reoxygenation

作　　者：董博[1] 杨常春[1] 王强[1] 张一[1] 李璐[1] 杨伊林[1] 周岱[2] 

机构地区：[1]苏州大学附属第三医院,江苏常州213003 [2]苏州大学附属第一医院

出　　处：《山东医药》2009年第2期12-14,共3页Shandong Medical Journal

摘　　要：目的观察IL-10对缺氧/复氧大鼠皮层神经元细胞内pH和游离Ca2+的影响,探讨IL-10的神经保护机制。方法取新生SD大鼠脑皮层进行原代皮层神经元培养。将神经元细胞分成A、B、C、D、E组,A组按常规培养,B、C、D、E组用PBS缓冲液冲洗3遍后,无血清DMEM缺氧培养2 h(95%N2,5%CO2),复氧6 h,C、D、E组分别在缺氧培养前即刻加入10、50、100 ug/L的IL-10。以Fura-2/AM和BCECF/AM作为细胞内Ca2+和H+的荧光指示剂,用荧光分光光度计检测神经元细胞内pH和Ca2+。结果A、B、C、D、E组pH值分别为7.18±0.074、6.27±0.058、6.41±0.036、6.72±0.103和6.98±0.031,Ca2+浓度分别为(98.12±18.74)、(178.86±46.09)、(157.53±38.17)、(131.53±24.69)和(119.64±27.69)nmol/L;A组pH值和Ca2+浓度与其他四组比较,P均<0.05;B组与C、D、E组比较,P均<0.05;D组与C组、E组与D组、E组与C组比较,P均<0.05。结论IL-10能抑制缺氧/复氧导致的神经元细胞内pH的下降和游离Ca2+的升高,这可能是其发挥神经保护作用的机制之一。Objective To detect the effect of IL-10 on the variation of intracellular pH and calcium ions in the cultured rat conical neurons following hypoxie and reoxygenation. Methods The intraeellular pH and calcium concentration were measured by fluorescence spectrophotometer. We used Fura-2/AM and BCECF/AM as a calcium and hydrogen indicator respectively. Results The results indicated that the calcium concentration significantly increased while the pH decreased during hypoxic and reoxygenation. IL-10 can reduce the free calcium concentration and inhibit the intracellular acidosis. The inhibitory effect is positively related to the IL-10 concentration. Conclusions IL-10 play neuroprotective effect partly via inhibiting the increase of calcium concentration and the intracellular acidosis during hypoxic and reoxygenation.

关 键 词：神经元 脑损伤 白细胞介素-IO PH 游离钙 

分 类 号：F364.4[经济管理—产业经济] R743[医药卫生—神经病学与精神病学]