促血管生成素-1对人胃癌细胞黏附和转移作用的研究  

Angiopoietin-1 promotes adhesion and metastasis of human gastric cancer cell line

作　　者：陈慧娟[1] 欧希龙[1] 孙为豪[1] 杨柳[1] 杭程[1] 刘丽[1] 

机构地区：[1]东南大学附属中大医院消化科,江苏南京210009

出　　处：《医学研究生学报》2009年第2期126-130,I0003,共6页Journal of Medical Postgraduates

基　　金：江苏省医学重点人才培养基金资助项目(批准号:R1207046)

摘　　要：目的:探讨促血管生成素-1(Ang-1)对人胃癌细胞中整合素β1(integrin β1)和CD44V6的作用,研究其对肿瘤细胞黏附和转移的影响及可能的作用机制。方法:利用腺病毒为载体,将已构建成功的、含Ang-1基因的重组腺病毒(Ad-Ang-1)瞬时转染人胃癌细胞株BGC-823,转染含绿色荧光蛋白基因的重组腺病毒(Ad-GFP)作为阴性对照,未转染的正常细胞作为空白对照,用细胞黏附法检测转染前后细胞与细胞外基质黏附率的改变,再分别用半定量RT-PCR和Western blot方法对3组细胞中整合素β1和CD44V6的mRNA和蛋白表达水平进行分析。结果:细胞黏附实验显示,转染Ad-Ang-1后,细胞与细胞外基质之间的黏附率明显增强(P<0.01);RT-PCR、Western blot结果显示整合素β1、CD44V6 mRNA和蛋白的表达在Ad-Ang-1组明显高于空白对照组及Ad-GFP组。结论:转染Ang-1能明显提高人胃癌细胞BGC-823中整合素β1、CD44V6 mRNA、蛋白的表达,从而促进肿瘤细胞的黏附和转移。Objective ： To evaluate the effects of Angiopoietin-1 on cell adhesion of human gastric cancer cell line BGC-823 and to investigate its influence on the expression of integrin β1 and CD44V6 of the cells. Methods： BGC-823 cells were transfected with Ad-Ang-1 transiently. Cells transfected transiently with Ad-GFP and untransfected cells were used as a negative control and a blank control group, respectively. The cell adhesion rate between the cells and extracellular matrix was presented by cell adhesion assay. The mRNA and protein expression of integrin β1 and CD44V6 were detected by RT-PCR and Western blot, respectively. Results： BGC-823 cells were successfully transfected. In the Ad-Ang-1 group, the adhesion rate increased significantly （P 〈 0.05）, and the mRNA and protein expression of integrin β1 and CD44V6 were higher than in the Ad-GFP and the blank control group （P 〈 0.05）. Conclusion ： Transfection of Ang-1 into human gastric cancer cell line BGC-823 can significantly increase the expressions of integrin β1 and CIM4V6, and hence promote the cell adhesion and metastasis to extracellular matrix.

关 键 词：胃癌 促血管生成素-1 整合素Β1 CD44V6 黏附 转移 

分 类 号：R735.2[医药卫生—肿瘤]