阿霉素心肌病兔模型心肌损害变化的研究  

Variation of Myocardial Damage in Dilated Cardiomypathy Rabbit Model Induced by Adriamycin

作　　者：苗海卫[1] 罗心平[2] 黄成磊[2] 王逸平[3] 孙伟康[3] 

机构地区：[1]复旦大学附属金山医院心内科,上海200540 [2]复旦大学附属华山医院心内科,上海200040 [3]中国科学院院上海药物研究所,上海201203

出　　处：《实验动物与比较医学》2009年第1期1-5,共5页Laboratory Animal and Comparative Medicine

摘　　要：目的研究阿霉素心肌病动物模型心肌损害的变化规律，为更好地使用这种模型做一探讨。方法普通级雄性新西兰兔32只，随机分为对照组（n=8）及改造模组（n=24）。其中正常组新西兰兔8只，经耳缘静脉注射等体积的生理盐水。模型组24只，经耳缘静脉注射生理盐水及盐酸阿霉素2mg／kg体重，每周1次，连续给药8周，建立新西兰兔扩张性心肌病（DCM）模型。对照组在第8周，模型组分别在给药第8周，第10周，第12周检测心超，B-型脑钠肽（BNP），血流动力学。然后处死做超微结构和病理学检查。结果模型组心脏超声检查，血流动力学检查都符合扩张性心肌病的变化。BNP和病理学检查8周模型组出现心肌损害，10周模型组损害最为严重，12周模型组损害较10周时有所缓解，变化明显（P〈0．05）。结论造模成功后2周心肌损害最严重，是使用本模型研究心衰的最佳时段。Objective To observe the rule of variation myocardial damage in adriamycin-induced dilated cardiomyopathy （DCM） in rabbit, and to determine the optimal time for further research. Methods Thrity-two rabbits were randomly assigned to control group（n=8） and model group （n=24）. The DCM model was induced by adriamycin, 2 ml/kg body weight via ear vein for 8 weeks, the control group was given 2 ml/kg body weight of saline. Echocardiogram and hemodynamics parameters were assessed and plasma BNP was measuered at the 8th,10th and 12th week after the injections to model group ,and at 8th week to control group. Then the pathological change of the myocardium and microstructure were observed. Results The results of hemodynamics, ultrastructure and echocardiogram were coincident to the change of DCM. The myocardial damage occurred at 8th week in model group, and was most serious at 10th week, and lessened at 12th week （P 〈0.05）. Conclusions AT two weeks after the model established, the myocardial damage is most serious. It＇s the optimal time for studying heart failure.

关 键 词：阿霉素 扩张性心肌病 心肌损伤 动物模型 

分 类 号：R-332[医药卫生] R965