KL-6黏蛋白在肝癌组织和血清中的表达及临床意义  

Expression and significance of KL-6 mucin in the tissue and serum of hepatoma

作　　者：许焕丽[1] 稻垣善则[2] 王凤山[1] 张克明[3] 国土典宏[2] 唐伟[1] 

机构地区：[1]山东大学药学院生化与生物技术药物研究所,济南250012 [2]日本东京大学医学部附属医院肝胆胰外科,113-8655 [3]解放军总医院全军肝胆外科研究所,北京100853

出　　处：《中华消化外科杂志》2009年第1期39-42,共4页Chinese Journal of Digestive Surgery

摘　　要：目的检测KL-6黏蛋白在不同类型肝癌患者癌组织和血清中的表达水平，并探讨其作为肿瘤标志物在临床诊断中应用的价值。方法采用免疫组织化学方法检测81例肝细胞癌（HCC）、21例胆管细胞癌（CO）、12例混合型肝细胞-胆管细胞癌（HCC-CC）和56例原发性结直肠癌肝转移（MLC）患者癌组织中KL-6黏蛋白的表达水平。用ELISA法测定其中34例HCC患者、8例CC患者、30例MLC患者和19例健康自愿者血清中KL-6黏蛋白的表达水平，并用t检验分析其结果。结果免疫组织化学分析结果表明，所有的CC患者和HCC-CC患者中，cc组织（12／12，100％）KL-6黏蛋白均呈阳性染色，而MLC患者的癌组织（56／56，100％）显示数个癌细胞或部分癌组织呈阳性染色。另外，在HCC和HCC-CC患者的HCC组织以及所有的非癌组织中KL-6黏蛋白没有发现阳性染色。CC患者的血清KL-6黏蛋白表达水平比健康自愿者、HCC患者和MLC患者高（t=5．58，5．34，4．00，P〈0．01）。MLC患者和健康自愿者的血清KL-6黏蛋白表达水平比较，差异有统计学意义（t=2．77，P〈0．01）。健康自愿者和HCC患者，以及MLC患者和HCC患者之间的血清KL-6黏蛋白表达水平比较，差异无统计学意义（t=2．03，1．89，P〉0．05）。结论KL．6黏蛋白在CC患者癌组织和血清中的表达水平均比HCC患者、MLC患者和健康自愿者高，可成为CC患者的特异性肿瘤标志物。Objective To detect the expression of KL-6 mucin in the tissue and serum of hepatoma in different hepatoma patients, and to investigate the value of KL-6 mucin as a tumor marker in the diagnosis of hepatoma. Methods The expression of KL-6 muein in the hepatoma tissues of 81 patients with hepatoeellular carcinoma （HCC）, 21 patients with cholangiocarcinoma （CC）, 12 patients with combined hepatocellular and cholangiocarcinoma （HCC-CC） and 56 patients with metastatic liver cancer （MLC） was detected by immunohisto-chemical analysis. The expression of KL-6 mucin in the serums of 34 HCC patients, 8 CC patients, 30 MLC patients and 19 healthy individuals was detected by enzyme-linked immunosorbent assay, and all the data were analyzed by t test. Results Expression of KL-6 mucin was detected in the cholangiocarcinoma tissues in all CC and HCC-CC patients. In several hepatoma cells and partial hepatoma tissues of patients with MLC, the expression of KL-6 mucin was detected. No expression of KL-6 mucin was detected in the hepatocellular carcinoma tissues and non-cancerous tissues of patients with HCC or HCC-CC. The serum levels of KL-6 mucin expression in CC patients were significantly higher than those in healthy individuals, HCC and MLC patients （ t = 5.58, 5.34, 4.00, P 〈0.01 ）. The difference of the serum levels of KL-6 mucin expression between MLC patients and healthy individuals had statistical significance （ t = 2.77, P 〈 0.01 ）. However, no significant difference in serum levels of KL-6 mucin expression was found between HCC patients and healthy individuals and between HCC patients and MLC patients （ t = 2.03, 1.89, P 〉 0.05）. Conclusion The expression of KL-6 mucin in CC patients is significantly higher than in patients with other types of hepatoma in both tissue and serum levels. Thus, KL-6 may be a useful new tumor marker for distinguishing CC from other types of hepatoma.

关 键 词：癌 肝细胞 KL-6黏蛋白 胆管细胞癌 转移性肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R563.13[医药卫生—临床医学]