大肠癌患者细胞因子的变化及其临床意义  

Clinical significance of the cytokines shift in patients of colonic cancer

作　　者：乔丽娟[1] 马克蓉[1] 麦羡霞[1] 赵晴[1] 李耀平[2] 

机构地区：[1]山西省肿瘤研究所免疫室,030013 [2]山西省肿瘤医院肛肠科

出　　处：《肿瘤研究与临床》2009年第2期98-100,共3页Cancer Research and Clinic

摘　　要：目的观察不同治疗阶段大肠癌患者T淋巴细胞亚群CD4^＋细胞中，Th1／Th2类因子水平的变化，为大肠癌的生物治疗提供依据。方法分离外周血淋巴细胞，经刺激增加细胞内因子表达，荧光标记特异性抗细胞因子单克隆抗体，特异性抗原抗体结合，用流式细胞术分析、测定特异性细胞因子表达水平。结果大肠癌患者在不同的治疗阶段，Th1类因子的变化较明显，差异均有统计学意义；Th2类因子变化无显著性。IFN-γ／IL-4的比值变化只在生物治疗前后差异有统计学意义。结论大肠癌患者在不同治疗阶段，Th1类因子水平的变化对生物治疗具有明显的指导意义；Th2类因子水平无指导意义。其中IFN-γ／IL-4比值的变化在生物治疗中具有较好的指导性，合理的生物治疗可逆转Th1／Th2因子的抗肿瘤抑制作用。Objective To observe the levels of Th1 and Th2 type cytokines of peripheral blood CD4^＋ T lymphocytes at different stage of treatment of colonic cancer, in order to find out the laboratory evidence of tumor immunotherapy. Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance the expression of cytokines. Then specific fluorescent-conjugated monoclonal antibodies were added to combine with its specific antigens. Cytokines were detected by flow cytometry. Results At different stage of treatment of colonic cancer, the levels of Thl type cytokines of each stage was significant.The levels of Th2 type eytokines at each stage were not significant. The IFN-γ//IL-4 ratio were significantly different during tumor immunotherapy. Conclusion At different stage of treatment of colonic cancer, the levels of Th1 type cytokines were obviously associated with treatment. The levels of Th2 type cytokines had no association with treatment. Among them, the changes of Th1/Th2 ratio （IFN-γ/IL-4） had more correlation with tumor immunotherapy. So the reasonable immunotherapy can reverse Th1/Th2 ratio and immune inhibition.

关 键 词：肠肿瘤 细胞因子 流式细胞术 

分 类 号：R735[医药卫生—肿瘤]