VEGF、bFGF、TGFβ1在非小细胞肺癌中的表达及临床意义  

Expression and Significance of Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor and Transforming Growth Factor β1 in Non-small Cell Lung Cancer

作　　者：姜久仰[1] 孟祥宁[2] 王柏春[1] 李春香[2] 禹亮[1] 

机构地区：[1]哈尔滨医科大学附属第一医院胸外科,150001 [2]哈尔滨医科大学医学遗传学研究室,150081

出　　处：《国际遗传学杂志》2009年第1期1-5,共5页International Journal of Genetics

基　　金：黑龙江省卫生厅科研项目（2004-079;2006-276）

摘　　要：目的探讨VEGF、bFGF、TGFβ1在非小细胞肺癌的表达及与其生物学行为特征的关系。方法应用链霉菌抗生素蛋白．过氧化物酶连结（streptavidin—perosidase，SP）免疫组化方法检测VEGF、bFGF、TGFβ1，及CD34在50例非小细胞肺癌的表达，并进行微血管密度计数，以18例癌旁组织为对照。结果VEGF、bFGF、TGFβ1的阳性表达率分别为68％、58％、60％，VEGF、bFGF、TGFβ1表达均与NSCLC的临床分期（χ^2值分别为4．535、3．864、3．852，P〈0．05）、淋巴结转移有关（χ^2值分别为4．235、3．876、4．151，P〈0．05），而TGFβ1表达还与组织学类型有关（χ^2=4．443，P〈0．05），鳞癌比腺癌表达率高。MVD表达与NSCLC的临床分期、淋巴结转移有关（t值分别为2．312和2．865，P〈0．05），与组织学类型、组织分化程度无关；且四者表达在癌组织与癌旁组织之间均有显著性统计学意义。VEGF、bFGF表达与MVD有关（t值分别为2．518和3．679，P〈0．05）；TGFβ1表达与MVD无显著关系。结论VEGF、bFGF、TGFβ1在NSCLC均有很好的表达，并且其表达与NSCLC的生长、进展、转移等生物学关系密切；VEGF、bFGF在肿瘤血管形成、转移中起重要作用，TGFβ1可能与机体免疫功能降低及肿瘤细胞免疫逃逸有关。Objective To evaluate the expression of vascular endothelial growth factor（ VEGF）, basic fibroblast growth factor（bFGF） and transforming growth factor betal （TGFβ1） in non-small cell lung cancer （NSCLC） and to investigate its relationship to histopathological features. Methods The expression of VEGF, bFGF, TGFβ1 and CD34 were examined in 50 specimens of NSCLC and 18 specimens of para-cancerous tissue by using SP immunohistochemistry staining. Assessment of microvessel density （MVD） was performed. Results The positive rate of VEGF,bFGF, TGFβ1 was 68%.The expression of VEGF was related to clinical classifica- tion and lymph nodes metastasis. The difference was significant among the groups （χ^2 = 4. 535 and 4. 235,P 〈 0.05）. The expression of VEGF had no significant relationship with some pathological characteristies such as hlstological types and pathological grade. The positive rate of bFGF was 58%. The expression of bFGF was related ）o clinical classification and lymph nodes metastasis. The difference was significant among the groups （χ^2 = 3. 864 and 3. 876,P 〈 0. 05 ）. The expression of bFGF has no significant relationship with some patholo- gical characteristics such as histological types, pathological grade. The positive rate of TGFβ1 was 60cA . The expression of TGFβ1 had significant relationship with some pathological characteristics such as histological types, clinical classification and lymph nodes metastasis. The difference was signifieant among the groups （ χ^2 = 3. 852,4. 151 and 4. 443 ,P 〈 0. 05 ）. The expression of TGFβ1 was not related to pathologieal grade. The expression of MVD was related to clinical classification and lymph nodes metastasis. The difference was significant among the groups （ t = 2. 312 and 2. 865, P 〈 0. 05 ）. The expression of MVD had no significant relationship with some pathological characteristics such as histological types and pathological grade. The four of all factors had closed statistical significance between cancer

关 键 词：非小细胞肺癌 血管内皮生长因子 碱性成纤维细胞生长因子 转化生长因子 微血管密度 

分 类 号：R734.2[医药卫生—肿瘤]