RANKL在成釉细胞瘤骨吸收机制中的作用  

Role of Receptor Activator of Nuclear Factor Kappa B Lignand in Bone Resorption Caused by Ameloblastoma

作　　者：钱永[1] 黄洪章[2] 张彬[2] 王建广[2] 

机构地区：[1]南昌大学第一附属医院口腔颌面外科,江西南昌330006 [2]中山大学附属第二医院口腔颌面外科,广东广州510120

出　　处：《现代生物医学进展》2009年第3期436-439,F0002,F0003,共6页Progress in Modern Biomedicine

基　　金：国家自然科学基金(30471 896);广东省自然科学基金(04300340)

摘　　要：目的:检测RANKL在成釉细胞瘤(ameloblastoma,AM)组织中的表达情况及探讨RANKL在AM骨吸收机制中的作用。方法:通过免疫组化方法检测RANKL在AM组织中的表达情况;通过建立AM细胞/新生大鼠骨细胞共培养体系,观察AM细胞诱导破骨细胞形成的活性,再以OPG(RANKL的抑制剂)进行干预,观察OPG对AM细胞诱导破骨细胞形成活性的影响。结果: RANKL在AM组织中有恒定的表达;AM细胞能够诱导新生大鼠骨细胞分化为成熟的破骨细胞,但此活性可被OPG明显抑制结论:AM细胞诱导破骨细胞形成可能是AM骨吸收过程中局部破骨细胞形成的重要来源和机制,而RANKL在此过程中发挥重要作用。Objective： To detect the expression of receptor activator of nuclear factor kappa B lignand（RANKL） in ameloblastoma tissues, and to explore the role of RANKL in the bone resorption caused by ameloblastoma. Methods： The expression of RANKL was measured by immunohistochemistry. Through erecting a co-culture system of ameloblastoma cells and bone cells of neonatal rats, the po- tential of ameloblastoma cells in inducing the osteoclastogenesis were observed. And then, the effect of OPG on the inducing activity of ameloblastoma cells was observed with OPG （the inhibitor of RANKL） intervention. Results： Constant expression of RANKL was found in ameloblastoma tissue. Ameloblastoma cells were able to induce the bone cells of neonatal rats to differentiate into mature osteoclasts, but the induction activity of ameloblastoma cells could be markedly inhibited by OPG. Conclusions： The osteoclastogenesis induced by ameloblastoma cells might be the important source and major mechanism of the locally osteoclast formation during the bone resorption process of ameloblastoma. RANKL played an essencial role in the osteoclastogenesis.

关 键 词：成釉细胞瘤 RANKL 共培养体系 破骨细胞形成 OPG 

分 类 号：R739.82[医药卫生—肿瘤]