EGFR/AKT信号转导通路在调控结肠癌LoVo细胞生物学行为中的作用研究  

Role of EGFR/AKT Pathway on the Biological Behavior of Human Colon Cancer Cells

作　　者：徐佳佳[1] 郭英[1] 李楠[1] 商延芳[1] 夏海鸣[1] 金治[1] 吴鹏[1] 黄培林[1] 

机构地区：[1]东南大学医学院病理与病理生理系,江苏南京210009

出　　处：《现代生物医学进展》2009年第3期440-443,F0003,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30400534)

摘　　要：目的:探讨EGFR/AKT(表皮生长因子受体/蛋白激酶B)信号转导通路在调控人结肠癌细胞株LoVo生物学行为中的作用机制方法:用EGFR抑制剂AG1478处理人结肠癌细胞LoVo;用免疫细胞化学法检测p-EGFR及p-AKT蛋白表达;用MTT法检测细胞的增殖;用流式细胞仪检测细胞的凋亡水平;用Transwell小室观察体外细胞迁移能力;用Transwell小室结合Matrigel胶检测肿瘤细胞侵袭能力结果:AG1478(20μmol/L)处理后,p-EGFR及p-AKT的表达水平明显降低(P<0.05),细胞的增殖、迁移和侵袭能力明显下降(P<0.05),细胞凋亡水平明显提高(P<0.05)。结论:EGFR与人结肠癌细胞LoVo的增殖、凋亡、迁移和侵袭性密切相关,并可能通过AKT信号转导通路调控人结肠癌的发展。Objective： To investigate the mechanism of epidermal growth factor receptor（EGFR）/Protein kinase B（PKB or AKT） signaling pathway on biological behavior of human colon cancer cell LoVo. Methods： LoVo cells were treated with AG1478. The expression of EGFR and AKT was detected by western blotting in LoVo cells. Cell proliferation assay and Matrixgel were used to examine the capability of proliferation, migration and invasion. FCM was used to examine the capability ofapoptosis. Results： After the treatment of AG 1478（20μmol/L）, the expression of p-EGFR and p-AKT were decreased remarkably （P〈0.05）. AG1478 inhibited significantly the capability of proliferation, migration and invasion in LoVo cells （P〈0.05）. The capability ofapoptosis was markedly improved （P〈0.05）. Conclusion： EGFR might play a key role on the capability of proliferation, apoptosis, migration and invasion in human colon cancer cell line LoVo. It could be partly related to the mechanism of AKT signaling pathway.

关 键 词：EGFR/AKT信号转导通路 AG1478 细胞增殖 细胞凋亡 细胞迁移 细胞侵袭 

分 类 号：R735.5[医药卫生—肿瘤]