苯醚甲环唑降解菌B2的分离、鉴定及其降解特性  

Isolation, identification and characteristics of a difenoconazole-degrading bacterial strain B2

作　　者：郑金伟[1] 何健[1] 王哲[1] 倪盈盈[1] 李顺鹏[1] 

机构地区：[1]南京农业大学生命科学学院,农业部农业环境微生物工程重点开放实验室,江苏南京210095

出　　处：《中国环境科学》2009年第1期42-46,共5页China Environmental Science

基　　金："十一五"国家科技支撑计划(2006BAD17D04);农业微生物菌种资源整理整合及共享试点项目(2005DKA21201-2)

摘　　要：从长期生产苯醚甲环唑的农药厂污泥中分离到1株能以苯醚甲环唑为唯一碳源生长的细菌，命名为B2.根据其生理生化特征和16SrRNA基因序列相似性分析，将该菌株鉴定为剑菌属（Ensifersp.）.菌株B2在24h内降解100mg/L的苯醚甲环唑的效率达85%以上.该菌株降解苯醚甲环唑的最适pH值为7.0，最适温度为30-35℃，降解速率与初始接种量呈正相关，与初始农药浓度呈负相关.基础盐培养基中，B2对初始浓度〉400mg/L的苯醚甲环唑几乎无降解作用.A bacteria strain B2 capable of utilizing difenoconazole as sole carbon source was isolated from sludge collected from a pesticide manufacturer which has been producing difenoconazole for many years. It ＇was identified as Ensifer sp. according to its physiological-biochemical properties and the analysis of its 16S rRNA gene sequence （GenBank Accession No. EU303300）. Above 85% of 100mg/L difenoconazole was degraded by strain B2 within 24h. The optimal pH and temperature for degradation were 7.0 and 30-35℃ respectively. The degrading rate showed a positive correlation with the amount of initial inoculum size while a negative correlation with the initial concentration of difenoconazole. No degradation occurred if the initial concentration of difenoconazole excessed 400mg/L in mineral salt medium.

关 键 词：苯醚甲环唑 剑菌属 降解特性 

分 类 号：X172[环境科学与工程—环境科学]