槲皮素诱导和增加HL-60白血病细胞对阿霉素的反应性  

Impact of Quercetin on adriamycin sensitivity and responsiveness in acute myeloid leukemic HL-60 cells

作　　者：李戈[1] 季丽娟[1] 黄建鸣[2] 

机构地区：[1]四川省人民医院儿科,成都610072 [2]四川省肿瘤医院

出　　处：《中国小儿血液与肿瘤杂志》2009年第1期12-15,共4页Journal of China Pediatric Blood and Cancer

基　　金：四川省科技厅重点科技项目(05JY029-039)

摘　　要：目的槲皮素(Quercetin),磷脂酰肌醇3激酶(P13K)和酪氨酸激酶(TPK)抑制剂。本研究为探索槲皮素对HL-60白血病细胞的生长抑制和增加阿霉素的细胞毒作用。方法采用MTT法分别测定不同浓度槲皮素,阿霉素以及槲皮素诱导下阿霉素对HL-60白血病细胞的生长抑制作用,比较其半数有效抑制浓度(IC50)。结果阿霉素利槲皮素均呈浓度依赖的抑制HL-60细胞的存活,其IC50分别为(0.152±0.035)mg/L和(31.881±5.742μmol/L;槲皮素(IC10,10μmol/L)的诱导作用显著增加了阿霉素对HL-60细胞的细胞毒作用,其IC50为(0.033±0.007)mg/L,较单一阿霉素增加了4.57倍(P<0.01)。结论槲皮素能明显抑制HL-60白血病细胞的生长,且可诱导HD-60白血病细胞对阿霉素的敏感性和细胞毒作用。槲皮素可作为一个增加白血病细胞对蒽环类化疗药物反应性的诱导剂。Objective To study the influences of quercetin, as phosphatidytinositol 3 kinase （PI3K） and tyrosine kinase inhibitor, on the growth of HL-60 leukemia cells and on the cytotoxicity of adriamycin. Methods The inhibitory effects of quercetin, adriamycin and adriamycin in co-treatment with quercetin on the growth of HL-60 leukemia cells were investigated by using MTT assay, and the IC50＇s were measured as a curvilinear regression equation. Results Quercetin and adriamycin showed dose-dependent inhibition of HL-60 leukemia cells survival, the IC50＇ s were （ 31.88 ± 5. 742） μmoL/L and （0. 152 ± 0. 035 ）mg/L respectively; Quercetin enhanced the cytotoxicity of adriamycin to HL-60 leukemia ceils significantly, and the IC50 of adriamycin in co-treatment with quercetin （ IC50, 10 μmol/L） declined to（0. 033 ±0. 007）mg/L, showing the cytotoxicity of adriamycin with a 4.75 fold increase as comparing to that with a single dose （P 〈 0.01 ）. Conclusions Quercetin could inhibit growth of HL-60 leukemia cell and induce the responsiveness of HL-60 leukemia cells to adriamycin. Quercetin can be used as an inducer increasing reactions of leukemia cells to therapeutic anticancer agents of anthracyclines.

关 键 词：白血病 槲皮素 阿霉素 P13K 酪氨酸激酶 

分 类 号：R733.7[医药卫生—肿瘤] R285.5[医药卫生—临床医学]