虎杖甙对家兔肺缺血/再灌注损伤肺组织TLR4和ICAM-1表达的影响  

THE REGULATIVE EFFECTS OF POLYDATIN ON TOLL-LIKE RECEPTOR 4 SIGNAL TRANSDUCTION PATHWAY IN LUNG ISCHEMIA/REPERFUSION INJURY IN RABBITS

作　　者：金晓凤[1] 徐正衸[1] 王万铁[1] 许益笑[1] 张晓隆[1] 

机构地区：[1]温州医学院病理生理学教研室,浙江温州325035

出　　处：《中国应用生理学杂志》2009年第1期41-44,共4页Chinese Journal of Applied Physiology

基　　金：浙江省卫生厅科研基金资助项目(SWS00021)

摘　　要：目的:研究家兔肺缺血/再灌注损伤时Toll样受体4(TLR4)信号转导通路变化及虎杖甙(PD)对其影响。方法:复制在体肺缺血/再灌注损伤模型。健康日本大耳白免30只,随机分为对照(C)组、缺血/再灌注(I/R)组、PD组。鲎试剂试管法检测血浆内毒素(ET);RT-PCR法检测肺组织TLR4、核因子-κBp65(NF-κBp65)和细胞间粘附分子-1(ICAM-1)mRNA表达;苏木素-伊红(HE)染色,光镜观察肺组织形态学变化。结果:I/R组、PD组与C组相比血浆ET无显著差异(均P>0.05)。L/R组肺组织TLR4、NF-κBp65及ICAM-1mRNA表达较C组显著升高(均P<0.01); PD组上述改变较L/R组明显下降,但仍高于C组(均P<0.01);光镜见PD组肺组织结构损伤明显轻于I/R组。结论:PD治疗可以下调肺组织TLR4、NF-κBp65表达,进而抑制ICAM-1等炎症介质的转录和分泌,减轻肺缺血/再灌注损伤。Aim： To investigate protective effects of polydatin（PD） during lung ischemia/reperfusion in rabbits and its potential inechanisms. Me thods： Rabbit lung modal of ischemia/reperfusion（I/R） injury was constituted in vivo. Thirty rabbits were divided into groups randomly： Control（ C）, I/R, PD group, respectively. Endotoxin（ET） in plasma was analyzed by End-point Chromogenic Assay, the expression of Toll-like receptor 4（TLR4） mRNA, nuclear factor（NF）-KBp65 mRNA, intracellular adhesion molecule-1 （ICAM-1） mRNA were measured by RT-PCR, the morphological changes of lung tissue were observed with hematoxylin-eosin （HE） staining. Results： There was no significant difference in ET concentration of plasma between groups （all of P 〉 0.05）. The expression of TLR-4 mRNA, NF-KBp65 mRNA and ICAM-1mRNA in I/ R group were significantly increased as compared to C group and PD group, while those expressions in PD group were evidently higher than those in C group（all of P 〈 0.01）. Light microscope sbow＇ed that the lung pathological injuries in PD group were obviously alleviated as compared to I/R group. Conclusion： PD might have a protective effect on lung ischemia/reperfusion injury by down-regulating TLR4 and NF-KB expression, then inhibiting the release of mediators of inflan,nation as ICAM- 1.

关 键 词：虎杖甙 肺 缺血/再灌注损伤 TOLL样受体4 核因子-kBp65 细胞间粘附分子-1 

分 类 号：R363[医药卫生—病理学]